{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sadahiro T"],"funding":["Japan Heart Foundation Research Grant","Senshin Medical Research Foundation","Nakatomi Foundation","NICHD NIH HHS","Keio University Medical Science Fund","Daiichi-Sankyo Foundation of Life Science","Research Center Network for Realization of Regenerative Medicine","Miyata Cardiac Research Promotion Foundation","JSPS","Takeda Science Foundation","Japan Agency for Medical Research and Development","Keio University Grant-in-Aid for Encouragement of Young Medical Scientists","Japan Society for the Promotion of Science"],"pagination":["382-395.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6190602"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(3)"],"pubmed_abstract":["The mesoderm arises from pluripotent epiblasts and differentiates into multiple lineages; however, the underlying molecular mechanisms are unclear. Tbx6 is enriched in the paraxial mesoderm and is implicated in somite formation, but its function in other mesoderms remains elusive. Here, using direct reprogramming-based screening, single-cell RNA-seq in mouse embryos, and directed cardiac differentiation in pluripotent stem cells (PSCs), we demonstrated that Tbx6 induces nascent mesoderm from PSCs and determines cardiovascular and somite lineage specification via its temporal expression. Tbx6 knockout in mouse PSCs using CRISPR/Cas9 technology inhibited mesoderm and cardiovascular differentiation, whereas transient Tbx6 expression induced mesoderm and cardiovascular specification from mouse"],"journal":["Cell stem cell"],"pubmed_title":["Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification."],"pmcid":["PMC6190602"],"funding_grant_id":["02-002-0022","17H04179","R01 HD086026","16K19426","18K08114","02-002-0017"],"pubmed_authors":["Fukuda K","Haginiwa S","Nishizono H","Muraoka N","Murano K","Iwasaki YW","Saito K","Ieda M","Andersen P","Tamura F","Fujita J","Kawamura Y","Uosaki H","Tani H","Oda M","Goshima N","Kwon C","Kojima H","Miyoshi H","Sadahiro T","Kurotsu S","Isomi M","Tohyama S"],"additional_accession":[]},"is_claimable":false,"name":"Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification.","description":"The mesoderm arises from pluripotent epiblasts and differentiates into multiple lineages; however, the underlying molecular mechanisms are unclear. Tbx6 is enriched in the paraxial mesoderm and is implicated in somite formation, but its function in other mesoderms remains elusive. Here, using direct reprogramming-based screening, single-cell RNA-seq in mouse embryos, and directed cardiac differentiation in pluripotent stem cells (PSCs), we demonstrated that Tbx6 induces nascent mesoderm from PSCs and determines cardiovascular and somite lineage specification via its temporal expression. Tbx6 knockout in mouse PSCs using CRISPR/Cas9 technology inhibited mesoderm and cardiovascular differentiation, whereas transient Tbx6 expression induced mesoderm and cardiovascular specification from mouse","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2026-05-02T10:38:19.051Z","creation":"2019-09-12T07:00:46Z"},"accession":"S-EPMC6190602","cross_references":{"pubmed":["30100166"],"doi":["10.1016/j.stem.2018.07.001"]}}