{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Palanichamy K"],"funding":["The Ohio State University Comprehensive Cancer Center","NIH/NCI","NCI NIH HHS"],"pagination":["281-292"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6197705"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["36"],"pubmed_abstract":["<h4>Background</h4>The goal of this study is to identify and characterize treatment resistant tumor initiating cells (TRTICs) using orthotopic xenografts.<h4>Methods</h4>TRTICs were enriched from GBM cell lines using mouse xenografts treated with fractionated doses of radiation and temozolomide. TRTICs were characterized by neurosphere clonogenicity and self-renewal, serial xenotransplantation, differentiation potential, and mRNA & miRNA transcriptomic profiling. We use an unbiased approach to identify antigens encoding TRTIC and glioma stem cells (GSC) populations. Co-culture experiments of TRTIC and differentiated cells were conducted to evaluate the reliance of TRTIC differentiation on the secretome of differentiated cells.<h4>Findings</h4>TRTICs acquire stem-like gene expression signat"],"journal":["EBioMedicine"],"pubmed_title":["Cells isolated from residual intracranial tumors after treatment express iPSC genes and possess neural lineage differentiation plasticity."],"pmcid":["PMC6197705"],"funding_grant_id":["1RC2CA148190","1R01CA188228","R01 CA188228","R01 CA108633","1R01CA108633","RC2 CA148190"],"pubmed_authors":["Ray-Chaudhury A","Chakravarti A","Litzenberg KT","Jacob JR","Palanichamy K"],"additional_accession":[]},"is_claimable":false,"name":"Cells isolated from residual intracranial tumors after treatment express iPSC genes and possess neural lineage differentiation plasticity.","description":"<h4>Background</h4>The goal of this study is to identify and characterize treatment resistant tumor initiating cells (TRTICs) using orthotopic xenografts.<h4>Methods</h4>TRTICs were enriched from GBM cell lines using mouse xenografts treated with fractionated doses of radiation and temozolomide. TRTICs were characterized by neurosphere clonogenicity and self-renewal, serial xenotransplantation, differentiation potential, and mRNA & miRNA transcriptomic profiling. We use an unbiased approach to identify antigens encoding TRTIC and glioma stem cells (GSC) populations. Co-culture experiments of TRTIC and differentiated cells were conducted to evaluate the reliance of TRTIC differentiation on the secretome of differentiated cells.<h4>Findings</h4>TRTICs acquire stem-like gene expression signat","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Oct","modification":"2026-04-30T14:30:26.597Z","creation":"2019-03-27T00:04:13Z"},"accession":"S-EPMC6197705","cross_references":{"pubmed":["30269995"],"doi":["10.1016/j.ebiom.2018.09.019"]}}