{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fritsch TE"],"funding":["Coordenação de Aperfeiçoamento de Pessoal de Nível Superior"],"pagination":["767"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6199787"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["<h4>Background</h4>Small RNAs (sRNAs) are noncoding molecules that regulate different cellular activities in several bacteria. The role of sRNAs in gene expression regulation is poorly characterized in the etiological agent of porcine enzootic pneumonia Mycoplasma hyopneumoniae. We performed a global analysis of the sRNAs, sRNA target genes and regulatory elements previously identified in their genome and analyzed the expression of some sRNAs and their target genes by quantitative RT-PCR (qPCR) in three different culture conditions.<h4>Results</h4>Seven of the 145 sRNA target genes are organized as monocistronic genes (mCs) while the other 138 sRNA target genes are organized into transcriptional units (TU). The identification of transcriptional regulatory elements (promoter motif, DNA repe"],"journal":["BMC genomics"],"pubmed_title":["Global analysis of sRNA target genes in Mycoplasma hyopneumoniae."],"pmcid":["PMC6199787"],"funding_grant_id":["23038.010041/2013-13"],"pubmed_authors":["Siqueira FM","Schrank IS","Fritsch TE"],"additional_accession":[]},"is_claimable":false,"name":"Global analysis of sRNA target genes in Mycoplasma hyopneumoniae.","description":"<h4>Background</h4>Small RNAs (sRNAs) are noncoding molecules that regulate different cellular activities in several bacteria. The role of sRNAs in gene expression regulation is poorly characterized in the etiological agent of porcine enzootic pneumonia Mycoplasma hyopneumoniae. We performed a global analysis of the sRNAs, sRNA target genes and regulatory elements previously identified in their genome and analyzed the expression of some sRNAs and their target genes by quantitative RT-PCR (qPCR) in three different culture conditions.<h4>Results</h4>Seven of the 145 sRNA target genes are organized as monocistronic genes (mCs) while the other 138 sRNA target genes are organized into transcriptional units (TU). The identification of transcriptional regulatory elements (promoter motif, DNA repe","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Oct","modification":"2026-05-02T02:45:23.408Z","creation":"2019-03-27T00:05:23Z"},"accession":"S-EPMC6199787","cross_references":{"pubmed":["30352553"],"doi":["10.1186/s12864-018-5136-5"]}}