{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["67(8)"],"submitter":["Levander S"],"funding":["Vetenskapsrådet","Cancerfonden"],"pubmed_abstract":["<h4>Objective</h4>HCV is characterised by its ability to establish chronic infection in hepatocytes and to replicate in the presence of an inflammation. We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells.<h4>Design</h4>A total of 5 million H-2<sup>b</sup> positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma cells), were injected subcutaneously into syngeneic H-2<sup>b</sup>-restricted mice. Kinetics of tumour growth, HCV RNA replication levels and HCV-specific immune responses were monitored. For immune monitoring, new H-2<sup>b</sup>-restricted cytotoxic T cell epit"],"journal":["Gut"],"pagination":["1525-1535"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6204962"],"repository":["biostudies-literature"],"pubmed_title":["Immune-mediated effects targeting hepatitis C virus in a syngeneic replicon cell transplantation mouse model."],"pmcid":["PMC6204962"],"pubmed_authors":["Levander S","Frelin L","Long G","Rupp D","Ahlen G","Bartenschlager R","Sallberg M","Holmstrom F"],"additional_accession":[]},"is_claimable":false,"name":"Immune-mediated effects targeting hepatitis C virus in a syngeneic replicon cell transplantation mouse model.","description":"<h4>Objective</h4>HCV is characterised by its ability to establish chronic infection in hepatocytes and to replicate in the presence of an inflammation. We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells.<h4>Design</h4>A total of 5 million H-2<sup>b</sup> positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma cells), were injected subcutaneously into syngeneic H-2<sup>b</sup>-restricted mice. Kinetics of tumour growth, HCV RNA replication levels and HCV-specific immune responses were monitored. For immune monitoring, new H-2<sup>b</sup>-restricted cytotoxic T cell epit","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Aug","modification":"2026-06-06T22:40:02.444Z","creation":"2019-03-27T00:06:06Z"},"accession":"S-EPMC6204962","cross_references":{"pubmed":["28646094"],"doi":["10.1136/gutjnl-2016-313579"]}}