biostudies-literatureUnknown67(8)Levander SVetenskapsrådetCancerfonden<h4>Objective</h4>HCV is characterised by its ability to establish chronic infection in hepatocytes and to replicate in the presence of an inflammation. We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells.<h4>Design</h4>A total of 5 million H-2^b positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma cells), were injected subcutaneously into syngeneic H-2^b-restricted mice. Kinetics of tumour growth, HCV RNA replication levels and HCV-specific immune responses were monitored. For immune monitoring, new H-2^b-restricted cytotoxic T cell epitGut1525-1535https://www.ebi.ac.uk/biostudies/studies/S-EPMC6204962biostudies-literatureImmune-mediated effects targeting hepatitis C virus in a syngeneic replicon cell transplantation mouse model.PMC6204962Levander SFrelin LLong GRupp DAhlen GBartenschlager RSallberg MHolmstrom FfalseImmune-mediated effects targeting hepatitis C virus in a syngeneic replicon cell transplantation mouse model.<h4>Objective</h4>HCV is characterised by its ability to establish chronic infection in hepatocytes and to replicate in the presence of an inflammation. We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells.<h4>Design</h4>A total of 5 million H-2^b positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma cells), were injected subcutaneously into syngeneic H-2^b-restricted mice. Kinetics of tumour growth, HCV RNA replication levels and HCV-specific immune responses were monitored. For immune monitoring, new H-2^b-restricted cytotoxic T cell epit2018-01-01T00:00:00Z2018 Aug2026-06-06T22:40:02.444Z2019-03-27T00:06:06ZS-EPMC62049622864609410.1136/gutjnl-2016-313579