biostudies-literatureWang SRobbers Research AwardsNIHNIGMS NIH HHS28-38https://www.ebi.ac.uk/biostudies/studies/S-EPMC6205512biostudies-literatureUnknown187Bacterial infections cause acute and chronic diseases. Antimicrobial resistance and aging-related immune weakness remain challenging in therapy of infectious diseases. Vaccines are however an alternative to prevent bacterial infections. Here we report a facile method to rapidly generate bacterium-membrane-formed nanovesicles as a vaccine using nitrogen cavitation. The vaccine is comprised of double-layered membrane vesicles (DMVs) characterized by cryo-TEM, biochemistry and proteomics, showing DMVs possess the integrity of bacterial membrane and contain a wide range of membrane proteins required for vaccination. In the mouse sepsis model induced by Pseudomonas aeruginosa, we found that DMVs can improve mouse survival after mice were immunized with DMVs. The increased adaptive immunity and BiomaterialsA facile approach for development of a vaccine made of bacterial double-layered membrane vesicles (DMVs).PMC6205512R01GM116823R01 GM11682317A-2950-9842Wang SGao JWang ZLi MWang LfalseA facile approach for development of a vaccine made of bacterial double-layered membrane vesicles (DMVs).Bacterial infections cause acute and chronic diseases. Antimicrobial resistance and aging-related immune weakness remain challenging in therapy of infectious diseases. Vaccines are however an alternative to prevent bacterial infections. Here we report a facile method to rapidly generate bacterium-membrane-formed nanovesicles as a vaccine using nitrogen cavitation. The vaccine is comprised of double-layered membrane vesicles (DMVs) characterized by cryo-TEM, biochemistry and proteomics, showing DMVs possess the integrity of bacterial membrane and contain a wide range of membrane proteins required for vaccination. In the mouse sepsis model induced by Pseudomonas aeruginosa, we found that DMVs can improve mouse survival after mice were immunized with DMVs. The increased adaptive immunity and 2018-01-01T00:00:00Z2018 Dec2025-04-04T01:42:44.031Z2020-05-21T20:15:48ZS-EPMC62055123029293910.1016/j.biomaterials.2018.09.042