{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bache N"],"funding":["EC | Horizon 2020 Framework Programme","Novo Nordisk Foundation Center for Protein Research","Novo Nordisk Fonden","Max-Planck-Gesellschaft"],"pagination":["2284-2296"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6210218"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(11)"],"pubmed_abstract":["To further integrate mass spectrometry (MS)-based proteomics into biomedical research and especially into clinical settings, high throughput and robustness are essential requirements. They are largely met in high-flow rate chromatographic systems for small molecules but these are not sufficiently sensitive for proteomics applications. Here we describe a new concept that delivers on these requirements while maintaining the sensitivity of current nano-flow LC systems. Low-pressure pumps elute the sample from a disposable trap column, simultaneously forming a chromatographic gradient that is stored in a long storage loop. An auxiliary gradient creates an offset, ensuring the re-focusing of the peptides before the separation on the analytical column by a single high-pressure pump. This simplif"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics."],"pmcid":["PMC6210218"],"funding_grant_id":["NNF15SA0018240","MPI","grant NNF14CC0001","PI Matthias Mann","grant agreement no 686547 MSmed project","PI Jesper Velgaard Olsen"],"pubmed_authors":["Doll S","Bache N","Geyer PE","Treit PV","Muller JB","Mann M","Olsen JV","Vorm O","Falkenby L","Meier F","Hoerning O","Paron I","Bekker-Jensen DB"],"additional_accession":[]},"is_claimable":false,"name":"A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics.","description":"To further integrate mass spectrometry (MS)-based proteomics into biomedical research and especially into clinical settings, high throughput and robustness are essential requirements. They are largely met in high-flow rate chromatographic systems for small molecules but these are not sufficiently sensitive for proteomics applications. Here we describe a new concept that delivers on these requirements while maintaining the sensitivity of current nano-flow LC systems. Low-pressure pumps elute the sample from a disposable trap column, simultaneously forming a chromatographic gradient that is stored in a long storage loop. An auxiliary gradient creates an offset, ensuring the re-focusing of the peptides before the separation on the analytical column by a single high-pressure pump. This simplif","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Nov","modification":"2026-05-01T15:27:12.963Z","creation":"2019-03-26T22:50:19Z"},"accession":"S-EPMC6210218","cross_references":{"pubmed":["30104208"],"doi":["10.1074/mcp.TIR118.000853"]}}