biostudies-literatureBache NEC | Horizon 2020 Framework ProgrammeNovo Nordisk Foundation Center for Protein ResearchNovo Nordisk FondenMax-Planck-Gesellschaft2284-2296https://www.ebi.ac.uk/biostudies/studies/S-EPMC6210218biostudies-literatureUnknown17(11)To further integrate mass spectrometry (MS)-based proteomics into biomedical research and especially into clinical settings, high throughput and robustness are essential requirements. They are largely met in high-flow rate chromatographic systems for small molecules but these are not sufficiently sensitive for proteomics applications. Here we describe a new concept that delivers on these requirements while maintaining the sensitivity of current nano-flow LC systems. Low-pressure pumps elute the sample from a disposable trap column, simultaneously forming a chromatographic gradient that is stored in a long storage loop. An auxiliary gradient creates an offset, ensuring the re-focusing of the peptides before the separation on the analytical column by a single high-pressure pump. This simplifMolecular & cellular proteomics : MCPA Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics.PMC6210218NNF15SA0018240MPIgrant NNF14CC0001PI Matthias Manngrant agreement no 686547 MSmed projectPI Jesper Velgaard OlsenDoll SBache NGeyer PETreit PVMuller JBMann MOlsen JVVorm OFalkenby LMeier FHoerning OParon IBekker-Jensen DBfalseA Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics.To further integrate mass spectrometry (MS)-based proteomics into biomedical research and especially into clinical settings, high throughput and robustness are essential requirements. They are largely met in high-flow rate chromatographic systems for small molecules but these are not sufficiently sensitive for proteomics applications. Here we describe a new concept that delivers on these requirements while maintaining the sensitivity of current nano-flow LC systems. Low-pressure pumps elute the sample from a disposable trap column, simultaneously forming a chromatographic gradient that is stored in a long storage loop. An auxiliary gradient creates an offset, ensuring the re-focusing of the peptides before the separation on the analytical column by a single high-pressure pump. This simplif2018-01-01T00:00:00Z2018 Nov2026-05-01T15:27:12.963Z2019-03-26T22:50:19ZS-EPMC62102183010420810.1074/mcp.TIR118.000853