{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhan Y"],"funding":["the National Program on Key Basic Research Project","the China National Tobacco Corporation","the Technical Innovation Special Fund of Hubei Province"],"pagination":["306"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6225680"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["<h4>Background</h4>Poly-γ-glutamic acid (γ-PGA) is a natural polymer with great potential applications in areas of agriculture, industry, and pharmaceutical. The biodiesel-derived glycerol can be used as an attractive feedstock for γ-PGA production due to its availability and low price; however, insufficient production of γ-PGA from glycerol is limitation.<h4>Results</h4>The metabolic pathway of <i>Bacillus licheniformis</i> WX-02 was rewired to improve the efficiency of glycerol assimilation and the supply of NADPH for γ-PGA synthesis. GlpK, GlpX, Zwf, and Tkt1 were found to be the key enzymes for γ-PGA synthesis using glycerol as a feedstock. Through combinational expression of these key enzymes, the γ-PGA titer increased to 19.20 ± 1.57 g/L, which was 1.50-fold of that of the wild-type "],"journal":["Biotechnology for biofuels"],"pubmed_title":["Rewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid in <i>Bacillus licheniformis</i>."],"pmcid":["PMC6225680"],"funding_grant_id":["2018ACA149","110201502018","No. 2015CB150505"],"pubmed_authors":["Cai D","Wen Z","Yi L","Ma X","Sheng B","Wang H","Shi J","Chen S","Yang S","Zhan Y"],"additional_accession":[]},"is_claimable":false,"name":"Rewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid in <i>Bacillus licheniformis</i>.","description":"<h4>Background</h4>Poly-γ-glutamic acid (γ-PGA) is a natural polymer with great potential applications in areas of agriculture, industry, and pharmaceutical. The biodiesel-derived glycerol can be used as an attractive feedstock for γ-PGA production due to its availability and low price; however, insufficient production of γ-PGA from glycerol is limitation.<h4>Results</h4>The metabolic pathway of <i>Bacillus licheniformis</i> WX-02 was rewired to improve the efficiency of glycerol assimilation and the supply of NADPH for γ-PGA synthesis. GlpK, GlpX, Zwf, and Tkt1 were found to be the key enzymes for γ-PGA synthesis using glycerol as a feedstock. Through combinational expression of these key enzymes, the γ-PGA titer increased to 19.20 ± 1.57 g/L, which was 1.50-fold of that of the wild-type ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-26T23:07:11.311Z","creation":"2019-03-27T00:08:20Z"},"accession":"S-EPMC6225680","cross_references":{"pubmed":["30455735"],"doi":["10.1186/s13068-018-1311-9"]}}