biostudies-literatureBenasutti HNIBIB NIH HHSNCATS NIH HHSNational Cancer InstituteNCI NIH HHSNational Institute of Biomedical Imaging and Bioengineering2747-2755https://www.ebi.ac.uk/biostudies/studies/S-EPMC6231230biostudies-literatureUnknown28(11)Opsonization (coating) of nanoparticles with complement C3 component is an important mechanism that triggers immune clearance and downstream anaphylactic and proinflammatory responses. The variability of complement C3 binding to nanoparticles in the general population has not been studied. We examined complement C3 binding to dextran superparamagnetic iron oxide nanoparticles (superparamagnetic iron oxide nanoworms, SPIO NWs, 58 and 110 nm) and clinically approved nanoparticles (carboxymethyl dextran iron oxide ferumoxytol (Feraheme, 28 nm), highly PEGylated liposomal doxorubicin (LipoDox, 88 nm), and minimally PEGylated liposomal irinotecan (Onivyde, 120 nm)) in sera from healthy human individuals. SPIO NWs had the highest variation in C3 binding (n = 47) between subjects, with a 15-30 foBioconjugate chemistryVariability of Complement Response toward Preclinical and Clinical Nanocarriers in the General Population.PMC6231230R01 CA194058R01 EB022040EB022040CA194058UL1 TR001082Simberg DWang GVu VPGroman EScheinman RBenasutti HSaba LfalseVariability of Complement Response toward Preclinical and Clinical Nanocarriers in the General Population.Opsonization (coating) of nanoparticles with complement C3 component is an important mechanism that triggers immune clearance and downstream anaphylactic and proinflammatory responses. The variability of complement C3 binding to nanoparticles in the general population has not been studied. We examined complement C3 binding to dextran superparamagnetic iron oxide nanoparticles (superparamagnetic iron oxide nanoworms, SPIO NWs, 58 and 110 nm) and clinically approved nanoparticles (carboxymethyl dextran iron oxide ferumoxytol (Feraheme, 28 nm), highly PEGylated liposomal doxorubicin (LipoDox, 88 nm), and minimally PEGylated liposomal irinotecan (Onivyde, 120 nm)) in sera from healthy human individuals. SPIO NWs had the highest variation in C3 binding (n = 47) between subjects, with a 15-30 fo2017-01-01T00:00:00Z2017 Nov2026-05-06T06:21:48.969Z2019-03-27T00:07:24ZS-EPMC62312302909058210.1021/acs.bioconjchem.7b00496