biostudies-literatureDogar AMSwiss National Science Foundatione0206823https://www.ebi.ac.uk/biostudies/studies/S-EPMC6231638biostudies-literatureUnknown13(11)Regulation of mRNA stability by RNA-protein interactions contributes significantly to quantitative aspects of gene expression. We have identified potential mRNA targets of the AU-rich element binding protein AUF1. Myc-tagged AUF1 p42 was induced in mouse NIH/3T3 cells and RNA-protein complexes isolated using anti-myc tag antibody beads. Bound mRNAs were analyzed with Affymetrix microarrays. We have identified 508 potential target mRNAs that were at least 3-fold enriched compared to control cells without myc-AUF1. 22.3% of the enriched mRNAs had an AU-rich cluster in the ARED Organism database, against 16.3% of non-enriched control mRNAs. The enrichment towards AU-rich elements was also visible by AREScore with an average value of 5.2 in the enriched mRNAs versus 4.2 in the control group. YPloS oneShort-lived AUF1 p42-binding mRNAs of RANKL and BCL6 have two distinct instability elements each.PMC62316383100-06543531003A_125193Ambrosini GHagenbuchle OKuhn LCSchmid CDGrisoni-Neupert BPaillusson ABucher PPradervand SDogar AMPauchard-Batschulat RRichman LfalseShort-lived AUF1 p42-binding mRNAs of RANKL and BCL6 have two distinct instability elements each.Regulation of mRNA stability by RNA-protein interactions contributes significantly to quantitative aspects of gene expression. We have identified potential mRNA targets of the AU-rich element binding protein AUF1. Myc-tagged AUF1 p42 was induced in mouse NIH/3T3 cells and RNA-protein complexes isolated using anti-myc tag antibody beads. Bound mRNAs were analyzed with Affymetrix microarrays. We have identified 508 potential target mRNAs that were at least 3-fold enriched compared to control cells without myc-AUF1. 22.3% of the enriched mRNAs had an AU-rich cluster in the ARED Organism database, against 16.3% of non-enriched control mRNAs. The enrichment towards AU-rich elements was also visible by AREScore with an average value of 5.2 in the enriched mRNAs versus 4.2 in the control group. Y2018-01-01T00:00:00Z20182026-05-06T06:22:41.42Z2019-03-27T00:08:22ZS-EPMC62316383041898110.1371/journal.pone.0206823