{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Spater T"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6234670"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["<h4>Background</h4>The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments (ad-MVF) may serve as vascularization units. In contrast to SVF single cells, they represent a mixture of intact arteriolar, capillary and venular vessel segments. Therefore, we herein hypothesized that the ad-MVF-based prevascularization of scaffolds is superior to the conventional SVF single cells-based approach.<h4>Results</h4>SVF single cells and ad-MVF were enzymatically isolated from epididymal fat pads of green fluorescent protein (GFP)<sup>+</sup> donor mice to assess their viability and cellular composition using fluorescence microscopy and flow cytometry. "],"journal":["Journal of biological engineering"],"pubmed_title":["Prevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments."],"pmcid":["PMC6234670"],"funding_grant_id":["LA 2682/7-1"],"pubmed_authors":["Spater T","Nickels RM","Laschke MW","Menger MD","Frueh FS"],"additional_accession":[]},"is_claimable":false,"name":"Prevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments.","description":"<h4>Background</h4>The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments (ad-MVF) may serve as vascularization units. In contrast to SVF single cells, they represent a mixture of intact arteriolar, capillary and venular vessel segments. Therefore, we herein hypothesized that the ad-MVF-based prevascularization of scaffolds is superior to the conventional SVF single cells-based approach.<h4>Results</h4>SVF single cells and ad-MVF were enzymatically isolated from epididymal fat pads of green fluorescent protein (GFP)<sup>+</sup> donor mice to assess their viability and cellular composition using fluorescence microscopy and flow cytometry. ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-26T07:17:28.869Z","creation":"2019-03-27T00:08:55Z"},"accession":"S-EPMC6234670","cross_references":{"pubmed":["30473729"],"doi":["10.1186/s13036-018-0118-3"]}}