biostudies-literatureSpater TDeutsche Forschungsgemeinschaft24https://www.ebi.ac.uk/biostudies/studies/S-EPMC6234670biostudies-literatureUnknown12<h4>Background</h4>The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments (ad-MVF) may serve as vascularization units. In contrast to SVF single cells, they represent a mixture of intact arteriolar, capillary and venular vessel segments. Therefore, we herein hypothesized that the ad-MVF-based prevascularization of scaffolds is superior to the conventional SVF single cells-based approach.<h4>Results</h4>SVF single cells and ad-MVF were enzymatically isolated from epididymal fat pads of green fluorescent protein (GFP)⁺ donor mice to assess their viability and cellular composition using fluorescence microscopy and flow cytometry. Journal of biological engineeringPrevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments.PMC6234670LA 2682/7-1Spater TNickels RMLaschke MWMenger MDFrueh FSfalsePrevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments.<h4>Background</h4>The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments (ad-MVF) may serve as vascularization units. In contrast to SVF single cells, they represent a mixture of intact arteriolar, capillary and venular vessel segments. Therefore, we herein hypothesized that the ad-MVF-based prevascularization of scaffolds is superior to the conventional SVF single cells-based approach.<h4>Results</h4>SVF single cells and ad-MVF were enzymatically isolated from epididymal fat pads of green fluorescent protein (GFP)⁺ donor mice to assess their viability and cellular composition using fluorescence microscopy and flow cytometry. 2018-01-01T00:00:00Z20182025-04-26T07:17:28.869Z2019-03-27T00:08:55ZS-EPMC62346703047372910.1186/s13036-018-0118-3