{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2018"],"submitter":["Dekowska A"],"funding":["Ministerstwo Nauki i Szkolnictwa Wyzszego"],"pubmed_abstract":["PCR-RFLP targeting of the 16S rDNA and <i>rpoB</i> genes, as well as the <i>vdc</i> region, was applied to identify and differentiate between the spoilage and non-spoilage <i>Alicyclobacillus</i> species. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while the <i>rpoB</i> gene and <i>vdc</i> gene cluster analysis also revealed that there were two major types among the <i>A. acidoterrestris</i> isolates, one similar to the reference strain <i>A. acidoterrestris</i> DSM 2498, and the other similar to the reference strain <i>A. acidoterrestris</i> ATCC 49025. Heterogeneity was also"],"journal":["BioMed research international"],"pagination":["9608756"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6236696"],"repository":["biostudies-literature"],"pubmed_title":["Genetic Heterogeneity of <i>Alicyclobacillus</i> Strains Revealed by RFLP Analysis of <i>vdc</i> Region and <i>rpoB</i> Gene."],"pmcid":["PMC6236696"],"pubmed_authors":["Niezgoda J","Dekowska A","Sokolowska B"],"additional_accession":[]},"is_claimable":false,"name":"Genetic Heterogeneity of <i>Alicyclobacillus</i> Strains Revealed by RFLP Analysis of <i>vdc</i> Region and <i>rpoB</i> Gene.","description":"PCR-RFLP targeting of the 16S rDNA and <i>rpoB</i> genes, as well as the <i>vdc</i> region, was applied to identify and differentiate between the spoilage and non-spoilage <i>Alicyclobacillus</i> species. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while the <i>rpoB</i> gene and <i>vdc</i> gene cluster analysis also revealed that there were two major types among the <i>A. acidoterrestris</i> isolates, one similar to the reference strain <i>A. acidoterrestris</i> DSM 2498, and the other similar to the reference strain <i>A. acidoterrestris</i> ATCC 49025. Heterogeneity was also","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-05T09:32:47.492Z","creation":"2019-03-27T00:10:29Z"},"accession":"S-EPMC6236696","cross_references":{"pubmed":["30515419"],"doi":["10.1155/2018/9608756"]}}