biostudies-literatureKageyama YNIA NIH HHSNINDS NIH HHSNIH HHS16895https://www.ebi.ac.uk/biostudies/studies/S-EPMC6237870biostudies-literatureUnknown8(1)Amyloid β (Aβ) plays a critical role in the pathogenesis of Alzheimer's disease. Nevertheless, its distribution and clearance before Aβ plaque formation needs to be elucidated. Using an optimized immunofluorescent staining method, we examined the distribution of Aβ in the post-mortem parietal cortex of 35 subjects, 30 to 65 years of age, APOE ε3/ε3, without AD lesions. We used 11A1, an antibody against an Aβ conformer which forms neurotoxic oligomers. 11A1 immunoreactivity (IR) was present in cortical neurons, pericapillary spaces, astrocytes and the extracellular compartment at 30 years of age. The percentage of neurons with 11A1 IR did not change with age, but the number and percentage of astrocytes with 11A1 IR gradually increased. Notably, the percentage of pericapillary spaces labeledScientific reportsAmyloid β toxic conformer has dynamic localization in the human inferior parietal cortex in absence of amyloid plaques.PMC6237870S10 OD016374P50 AG005146K22 NS092688R21 AG055844P50 AG047266Pletnikova OTroncoso JCMurakami KIrie YAn YIrie KMartin LJRudow GLResnick SMKageyama YSaito AFowler DRfalseAmyloid β toxic conformer has dynamic localization in the human inferior parietal cortex in absence of amyloid plaques.Amyloid β (Aβ) plays a critical role in the pathogenesis of Alzheimer's disease. Nevertheless, its distribution and clearance before Aβ plaque formation needs to be elucidated. Using an optimized immunofluorescent staining method, we examined the distribution of Aβ in the post-mortem parietal cortex of 35 subjects, 30 to 65 years of age, APOE ε3/ε3, without AD lesions. We used 11A1, an antibody against an Aβ conformer which forms neurotoxic oligomers. 11A1 immunoreactivity (IR) was present in cortical neurons, pericapillary spaces, astrocytes and the extracellular compartment at 30 years of age. The percentage of neurons with 11A1 IR did not change with age, but the number and percentage of astrocytes with 11A1 IR gradually increased. Notably, the percentage of pericapillary spaces labeled2018-01-01T00:00:00Z2018 Nov2025-04-05T12:27:51.976Z2019-03-27T00:08:49ZS-EPMC62378703044297810.1038/s41598-018-35004-3