biostudies-literatureWalsh TGBritish Heart Foundatione24https://www.ebi.ac.uk/biostudies/studies/S-EPMC6241850biostudies-literatureUnknown1(1)Platelets are classically known for their roles in bleeding control and occlusive thrombus formation causing ischaemic tissue damage. Recently non-classical roles for platelets have been described, many of which may be mediated by the heterogeneous cargo that platelets secrete from granular stores upon activation. Using an in vitro model of ischaemic injury to ventricular cardiomyocytes, we observed that platelets, through secreted factors, delayed the rate of cardiomyocyte death during ischaemia. This protective effect appeared independent of platelet dense granule cargo, but required α-granule components stromal cell-derived factor (SDF)-1α and transforming growth factor (TGF) β1. Protein kinase C (PKC) activity within cardiomyocytes was responsible for mediating the protective signals iTH open : companion journal to thrombosis and haemostasisPlatelets Protect Cardiomyocytes from Ischaemic Damage.PMC6241850PG/13/14/30023RG/15/16/31758RG/10/006/28299Poole AWWalsh TGfalsePlatelets Protect Cardiomyocytes from Ischaemic Damage.Platelets are classically known for their roles in bleeding control and occlusive thrombus formation causing ischaemic tissue damage. Recently non-classical roles for platelets have been described, many of which may be mediated by the heterogeneous cargo that platelets secrete from granular stores upon activation. Using an in vitro model of ischaemic injury to ventricular cardiomyocytes, we observed that platelets, through secreted factors, delayed the rate of cardiomyocyte death during ischaemia. This protective effect appeared independent of platelet dense granule cargo, but required α-granule components stromal cell-derived factor (SDF)-1α and transforming growth factor (TGF) β1. Protein kinase C (PKC) activity within cardiomyocytes was responsible for mediating the protective signals i2017-01-01T00:00:00Z2017 Jun2025-04-04T08:41:15.712Z2019-03-27T00:08:45ZS-EPMC62418503046504010.1055/s-0037-1603928