{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rothenberg DA"],"funding":["Swiss National Science Foundation","NIEHS NIH HHS","NCI NIH HHS","NIH","National Research Foundation of Singapore","MIT Center for Precision Cancer Medicine"],"pagination":["367-381"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6249402"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC) and bio-orthogonal non-canonical amino acid tagging (BONCAT) to label newly synthesized proteins with azidohomoalanine (Aha), thus enabling high temporal resolution across multiple conditions in a single analysis. MITNCAT quantification of protein synthesis rates following induction of the unfolded protein response revealed global down-regulation of protein synthesis, with stronger down-regulation of glycolytic and protein synthesis machinery proteins, but up-regulation of several key chaperones. Waves of temporally distinct protein synthesis were observed in response to epidermal growth factor, with altered synthesis detectable in the first 15 "],"journal":["iScience"],"pubmed_title":["A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth."],"pmcid":["PMC6249402"],"funding_grant_id":["CA014051","ES024615","CA096504","P01 CA026731","P30 ES002109","CA210180","ES026856","ES002109","U54 CA210180","P30 CA014051","P2SKP3_174681","CA026731","R01 CA096504","R01 ES026856","174681","P42 ES027707","R01 ES024615"],"pubmed_authors":["White FM","Rothenberg DA","Huber SM","Begley TJ","Dedon PC","Taliaferro JM"],"additional_accession":[]},"is_claimable":false,"name":"A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth.","description":"To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC) and bio-orthogonal non-canonical amino acid tagging (BONCAT) to label newly synthesized proteins with azidohomoalanine (Aha), thus enabling high temporal resolution across multiple conditions in a single analysis. MITNCAT quantification of protein synthesis rates following induction of the unfolded protein response revealed global down-regulation of protein synthesis, with stronger down-regulation of glycolytic and protein synthesis machinery proteins, but up-regulation of several key chaperones. Waves of temporally distinct protein synthesis were observed in response to epidermal growth factor, with altered synthesis detectable in the first 15 ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Nov","modification":"2025-04-05T12:59:46.586Z","creation":"2019-03-27T00:10:00Z"},"accession":"S-EPMC6249402","cross_references":{"pubmed":["30466063"],"doi":["10.1016/j.isci.2018.11.004"]}}