biostudies-literatureWang Xthe innovation and strengthening project of Guangdong Ocean UniversityNatural Science Foundation of ChongqingGuangdong Science and Technology Department318https://www.ebi.ac.uk/biostudies/studies/S-EPMC6249879biostudies-literatureUnknown11<h4>Background</h4>Metabolic engineering has emerged as a potential strategy for improving microalgal lipid content through targeted changes to lipid metabolic networks. However, the intricate nature of lipogenesis has impeded metabolic engineering. Therefore, it is very important to identify the crucial metabolic nodes and develop strategies to exploit multiple genes for transgenesis. In an attempt to unravel the microalgal triacylglycerol (TAG) pathway, we overexpressed two key lipogenic genes, glycerol-3-phosphate acyltransferase (GPAT1) and lysophosphatidic acid acyltransferase (LPAT1), in oleaginous <i>Phaeodactylum tricornutum</i> and determined their roles in microalgal lipogenesis.<h4>Results</h4>Engineered <i>P. tricornutum</i> strains showed enhanced growth and photosynthetic effBiotechnology for biofuelsDual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in <i>Phaeodactylum tricornutum</i>.PMC6249879GDOU2013010203415761232018A0303131644157613241725002GDOU2014050201GDOU2013050201416714632016A0303120042014A030308010Balamurugan SYang WDWang XDong HPWei WLi HYLiu JSfalseDual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in <i>Phaeodactylum tricornutum</i>.<h4>Background</h4>Metabolic engineering has emerged as a potential strategy for improving microalgal lipid content through targeted changes to lipid metabolic networks. However, the intricate nature of lipogenesis has impeded metabolic engineering. Therefore, it is very important to identify the crucial metabolic nodes and develop strategies to exploit multiple genes for transgenesis. In an attempt to unravel the microalgal triacylglycerol (TAG) pathway, we overexpressed two key lipogenic genes, glycerol-3-phosphate acyltransferase (GPAT1) and lysophosphatidic acid acyltransferase (LPAT1), in oleaginous <i>Phaeodactylum tricornutum</i> and determined their roles in microalgal lipogenesis.<h4>Results</h4>Engineered <i>P. tricornutum</i> strains showed enhanced growth and photosynthetic eff2018-01-01T00:00:00Z20182026-05-05T22:14:53.612Z2019-03-27T00:09:59ZS-EPMC62498793047966310.1186/s13068-018-1317-3