biostudies-literatureResman FVetenskapsrådetNIAID NIH HHSHjärt-Lungfonden (SE)Hjärt-Lungfonden592https://www.ebi.ac.uk/biostudies/studies/S-EPMC6249890biostudies-literatureUnknown18(1)<h4>Background</h4>Several different IgA-proteases exist in Haemophilus influenzae. The variants have been suggested to play differential roles in pathogenesis, but there is limited information on their distribution in clinical isolates. The objective of this study was to investigate the distribution of IgA-protease genotypes in H. influenzae and assess the association between IgA-protease genotype and type of clinical infection.<h4>Methods</h4>We performed PCR-screening of the IgA-protease gene variants in two cohorts of clinical H. influenzae. The first cohort consisted of 177 isolates from individuals with respiratory tract infection in January 2010, 2011 and 2012. Information on age, gender and clinical infection was available in this cohort. The second cohort comprised 53 isolates, inBMC infectious diseasesDifferential distribution of IgA-protease genotypes in mucosal and invasive isolates of Haemophilus influenzae in Sweden.PMC624989020150697K2015-57X-03163-43-4R01 AI019641Resman FManat GLindh VRiesbeck KMurphy TFfalseDifferential distribution of IgA-protease genotypes in mucosal and invasive isolates of Haemophilus influenzae in Sweden.<h4>Background</h4>Several different IgA-proteases exist in Haemophilus influenzae. The variants have been suggested to play differential roles in pathogenesis, but there is limited information on their distribution in clinical isolates. The objective of this study was to investigate the distribution of IgA-protease genotypes in H. influenzae and assess the association between IgA-protease genotype and type of clinical infection.<h4>Methods</h4>We performed PCR-screening of the IgA-protease gene variants in two cohorts of clinical H. influenzae. The first cohort consisted of 177 isolates from individuals with respiratory tract infection in January 2010, 2011 and 2012. Information on age, gender and clinical infection was available in this cohort. The second cohort comprised 53 isolates, in2018-01-01T00:00:00Z2018 Nov2026-07-16T08:03:53.595Z2019-03-27T00:09:01ZS-EPMC62498903046640710.1186/s12879-018-3464-3