biostudies-literatureUnknown13Alimoradi H<h4>Purpose</h4>Anticancer drug delivery systems are often limited by hurdles, such as off-target distribution, slow cellular internalization, limited lysosomal escape, and drug resistance. To overcome these limitations, we have developed a stable nitric oxide (NO)-releasing nanoparticle (polystyrene-maleic acid [SMA]-<i>tert</i>-dodecane S-nitrosothiol [tDodSNO]) with the aim of enhancing the anticancer properties of doxorubicin (Dox) and a Dox-loaded nanoparticle (SMA-Dox) carrier.<h4>Materials and methods</h4>Effects of SMA-tDodSNO and/or in combination with Dox or SMA-Dox on cell viability, apoptosis, mitochondrial membrane potential, lysosomal membrane permeability, tumor tissue, and tumor growth were studied using in vitro and in vivo model of triple-negative breast cancer (TNBC). InInternational journal of nanomedicine7771-7787https://www.ebi.ac.uk/biostudies/studies/S-EPMC6251458biostudies-literatureNitric oxide-releasing nanoparticles improve doxorubicin anticancer activity.PMC6251458Barzegar-Fallah AAlshaibani LAlimoradi HGreish KPittala VfalseNitric oxide-releasing nanoparticles improve doxorubicin anticancer activity.<h4>Purpose</h4>Anticancer drug delivery systems are often limited by hurdles, such as off-target distribution, slow cellular internalization, limited lysosomal escape, and drug resistance. To overcome these limitations, we have developed a stable nitric oxide (NO)-releasing nanoparticle (polystyrene-maleic acid [SMA]-<i>tert</i>-dodecane S-nitrosothiol [tDodSNO]) with the aim of enhancing the anticancer properties of doxorubicin (Dox) and a Dox-loaded nanoparticle (SMA-Dox) carrier.<h4>Materials and methods</h4>Effects of SMA-tDodSNO and/or in combination with Dox or SMA-Dox on cell viability, apoptosis, mitochondrial membrane potential, lysosomal membrane permeability, tumor tissue, and tumor growth were studied using in vitro and in vivo model of triple-negative breast cancer (TNBC). In2018-01-01T00:00:00Z20182025-04-05T12:59:55.512Z2019-03-27T00:11:27ZS-EPMC62514583053845810.2147/IJN.S187089