biostudies-literatureKo YSNational Research Foundation of KoreaE3350https://www.ebi.ac.uk/biostudies/studies/S-EPMC6274837biostudies-literatureUnknown19(11)The cardiac glycosides oleandrin and odoroside A, polyphenolic monomer compounds extracted from <i>Nerium oleander</i>, have been found to have antitumor effects on various tumors at low doses. However, the mechanisms of anticancer effects of oleandrin and odoroside A are not well known. Therefore, in this study, we aimed to investigate the anticancer effects of oleandrin and odoroside A and their associated mechanisms in highly metastatic MDA-MB-231 breast cancer cells and radiotherapy-resistant (RT-R) MDA-MB-231 cells. Our results showed that oleandrin and odoroside A dose-dependently decreased the colony formation and the invasion of both cell lines at nanomolar ranges. Furthermore, oleandrin (50 nM) and odoroside A (100 nM) reduced octamer-binding transcription factor 3/4 (OCT3/4) and International journal of molecular sciencesOleandrin and Its Derivative Odoroside A, Both Cardiac Glycosides, Exhibit Anticancer Effects by Inhibiting Invasion via Suppressing the STAT-3 Signaling Pathway.PMC62748372015R1A1A3A040010292018R1A2B6001786Rugira TJin HKo YSKim HJPark SWfalseOleandrin and Its Derivative Odoroside A, Both Cardiac Glycosides, Exhibit Anticancer Effects by Inhibiting Invasion via Suppressing the STAT-3 Signaling Pathway.The cardiac glycosides oleandrin and odoroside A, polyphenolic monomer compounds extracted from <i>Nerium oleander</i>, have been found to have antitumor effects on various tumors at low doses. However, the mechanisms of anticancer effects of oleandrin and odoroside A are not well known. Therefore, in this study, we aimed to investigate the anticancer effects of oleandrin and odoroside A and their associated mechanisms in highly metastatic MDA-MB-231 breast cancer cells and radiotherapy-resistant (RT-R) MDA-MB-231 cells. Our results showed that oleandrin and odoroside A dose-dependently decreased the colony formation and the invasion of both cell lines at nanomolar ranges. Furthermore, oleandrin (50 nM) and odoroside A (100 nM) reduced octamer-binding transcription factor 3/4 (OCT3/4) and 2018-01-01T00:00:00Z2018 Oct2026-07-09T12:06:30.271Z2025-05-18T13:33:43.949ZS-EPMC62748373037317110.3390/ijms19113350