biostudies-literatureUnknown15(2)Bunimov NInduction of Prostaglandin Endoperoxide H Synthase-1 (PGHS-1) gene has been previously documented in a few studies during events such as development and cellular differentiation. However, molecular mechanisms governing the regulation of PGHS-1 gene expression and contributing to changes in protein levels are poorly understood. Using the MEG-01 cell model of PGHS-1 gene induction, our laboratory has previously demonstrated that the 5'UTR and the first two exons of PGHS-1 mRNA had a significant impact on decreasing the translational efficiency of a reporter gene and suggested that the presence of a secondary structure is required for conservation of this activity. This 5'end of PGHS-1 mRNA sequence has also been shown to associate with nucleolin protein. In the current study, we set to invesCellular & molecular biology letters196-214https://www.ebi.ac.uk/biostudies/studies/S-EPMC6275937biostudies-literatureCharacterization of proteins associating with 5' terminus of PGHS-1 mRNA.PMC6275937Laneuville OBunimov NfalseCharacterization of proteins associating with 5' terminus of PGHS-1 mRNA.Induction of Prostaglandin Endoperoxide H Synthase-1 (PGHS-1) gene has been previously documented in a few studies during events such as development and cellular differentiation. However, molecular mechanisms governing the regulation of PGHS-1 gene expression and contributing to changes in protein levels are poorly understood. Using the MEG-01 cell model of PGHS-1 gene induction, our laboratory has previously demonstrated that the 5'UTR and the first two exons of PGHS-1 mRNA had a significant impact on decreasing the translational efficiency of a reporter gene and suggested that the presence of a secondary structure is required for conservation of this activity. This 5'end of PGHS-1 mRNA sequence has also been shown to associate with nucleolin protein. In the current study, we set to inves2010-01-01T00:00:00Z2010 Jun2026-05-06T00:32:47.602Z2025-05-18T13:35:11.282ZS-EPMC62759372011200110.2478/s11658-010-0005-5