{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(92)"],"submitter":["Yanagihara K"],"pubmed_abstract":["Poorly differentiated neuroendocrine carcinoma of the duodenum (D-NEC) is a rare cancer with poor prognosis. However, a D-NEC cell line has not yet been established to study the disease. We established a cell line, TCC-NECT-2, from the ascites tumor of a 59-year-old male Japanese patient with D-NEC. TCC-NECT-2 was positive for neuroendocrine markers, chromogranin A (CGA), cluster of differentiation 56 (CD56/NCAM), synaptophysin (SYN/p38), and neuron specific enolase (NSE). Cells exhibited retinoblastoma (RB) protein loss. Orthotopic implantation of TCC-NECT-2 cells into nu/nu mice resulted in tumor formation (incidence = 83.3%) with neuroendocrine characteristics, metastasis, and weight loss. BRAF<sup>V600E</sup> and TP53 mutations and C-MYC gene amplification were also observed in TCC-NEC"],"journal":["Oncotarget"],"pagination":["36503-36514"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6284856"],"repository":["biostudies-literature"],"pubmed_title":["Establishment of a novel cell line from a rare human duodenal poorly differentiated neuroendocrine carcinoma."],"pmcid":["PMC6284856"],"pubmed_authors":["Kubo T","Yanagihara K","Mihara K","Seyama T","Yokozaki H","Kuwata T","Ochiai A"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of a novel cell line from a rare human duodenal poorly differentiated neuroendocrine carcinoma.","description":"Poorly differentiated neuroendocrine carcinoma of the duodenum (D-NEC) is a rare cancer with poor prognosis. However, a D-NEC cell line has not yet been established to study the disease. We established a cell line, TCC-NECT-2, from the ascites tumor of a 59-year-old male Japanese patient with D-NEC. TCC-NECT-2 was positive for neuroendocrine markers, chromogranin A (CGA), cluster of differentiation 56 (CD56/NCAM), synaptophysin (SYN/p38), and neuron specific enolase (NSE). Cells exhibited retinoblastoma (RB) protein loss. Orthotopic implantation of TCC-NECT-2 cells into nu/nu mice resulted in tumor formation (incidence = 83.3%) with neuroendocrine characteristics, metastasis, and weight loss. BRAF<sup>V600E</sup> and TP53 mutations and C-MYC gene amplification were also observed in TCC-NEC","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Nov","modification":"2026-07-16T16:37:59.378Z","creation":"2019-03-27T00:12:52Z"},"accession":"S-EPMC6284856","cross_references":{"pubmed":["30559933"],"doi":["10.18632/oncotarget.26367"]}}