biostudies-literatureUnknown6(1)Truxova IA high density of tumor-infiltrating CD8⁺ T cells and CD20⁺ B cells correlates with prolonged survival in patients with a wide variety of human cancers, including high-grade serous ovarian carcinoma (HGSC). However, the potential impact of mature dendritic cells (DCs) in shaping the immune contexture of HGSC, their role in the establishment of T cell-dependent antitumor immunity, and their potential prognostic value for HGSC patients remain unclear. We harnessed immunohistochemical tests and biomolecular analyses to demonstrate that a high density of tumor-infiltrating DC-LAMP⁺ DCs is robustly associated with an immune contexture characterized by T_H1 polarization and cytotoxic activity. We showed that both mature DCs and CD20⁺ B cells play a critical role in the generation of a clinically-favorable cytotoxic immune response in HGSC microenvironment. In line with this notion, robust tumor infiltration by both DC-LAMP⁺ DCs and CD20⁺ B cells was associated with most favorable overall survival in two independent cohorts of chemotherapy-naïve HGSC patients. Our findings suggest that the presence of mature, DC-LAMP⁺ DCs in the tumor microenvironment may represent a novel, powerful prognostic biomarker for HGSC patients that reflects the activation of clinically-relevant anticancer immunity.Journal for immunotherapy of cancer139https://www.ebi.ac.uk/biostudies/studies/S-EPMC6288908biostudies-literatureMature dendritic cells correlate with favorable immune infiltrate and improved prognosis in ovarian carcinoma patients.PMC6288908Ryska AHensler MRob LSautes-Fridman CFucikova JSkapa PPecen LTruxova IPraznovec IKodet RSpisek RKasikova LBrtnicky TGoc JGalluzzi LBelicova LHalaska MJFridman WHLaco JSalkova EfalseMature dendritic cells correlate with favorable immune infiltrate and improved prognosis in ovarian carcinoma patients.A high density of tumor-infiltrating CD8⁺ T cells and CD20⁺ B cells correlates with prolonged survival in patients with a wide variety of human cancers, including high-grade serous ovarian carcinoma (HGSC). However, the potential impact of mature dendritic cells (DCs) in shaping the immune contexture of HGSC, their role in the establishment of T cell-dependent antitumor immunity, and their potential prognostic value for HGSC patients remain unclear. We harnessed immunohistochemical tests and biomolecular analyses to demonstrate that a high density of tumor-infiltrating DC-LAMP⁺ DCs is robustly associated with an immune contexture characterized by T_H1 polarization and cytotoxic activity. We showed that both mature DCs and CD20⁺ B cells play a critical role in the generation of a clinically-favorable cytotoxic immune response in HGSC microenvironment. In line with this notion, robust tumor infiltration by both DC-LAMP⁺ DCs and CD20⁺ B cells was associated with most favorable overall survival in two independent cohorts of chemotherapy-naïve HGSC patients. Our findings suggest that the presence of mature, DC-LAMP⁺ DCs in the tumor microenvironment may represent a novel, powerful prognostic biomarker for HGSC patients that reflects the activation of clinically-relevant anticancer immunity.2018-01-01T00:00:00Z2018 Dec2026-05-05T22:23:34.277Z2019-03-27T00:11:53ZS-EPMC62889083052666710.1186/s40425-018-0446-3