{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suraneni PK"],"funding":["NIDDK NIH HHS","NHLBI NIH HHS","NCI NIH HHS","NIH HHS"],"pagination":["3540-3552"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6290100"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(23)"],"pubmed_abstract":["Megakaryocyte (MK) migration from the bone marrow periosteal niche toward the vascular niche is a prerequisite for proplatelet extension and release into the circulation. The mechanism for this highly coordinated process is poorly understood. Here we show that dynasore (DNSR), a small-molecule inhibitor of dynamins (DNMs), or short hairpin RNA knockdown of DNM2 and DNM3 impairs directional migration in a human MK cell line or MKs derived from cultured CD34<sup>+</sup> cells. Because cell migration requires actin cytoskeletal rearrangements, we measured actin polymerization and the activity of cytoskeleton regulator RhoA and found them to be decreased after inhibition of DNM2 and DNM3. Because SDF-1α is important for hematopoiesis, we studied the expression of its receptor CXCR4 in DNSR-tre"],"journal":["Blood advances"],"pubmed_title":["Dynamins 2 and 3 control the migration of human megakaryocytes by regulating CXCR4 surface expression and ITGB1 activity."],"pmcid":["PMC6290100"],"funding_grant_id":["R01 DK098812","T32 CA080621","S10 OD011996","R01 HL080052","R01 HL112792","P30 CA060553","R21 HL106462","K08 HL114871"],"pubmed_authors":["Liu H","Wickrema A","Hession MJ","Eklund EA","Crispino JD","Debili N","Ishaq R","Corey SJ","Awomolo A","Hasan S","Shah C","Chen Y","Suraneni PK"],"additional_accession":[]},"is_claimable":false,"name":"Dynamins 2 and 3 control the migration of human megakaryocytes by regulating CXCR4 surface expression and ITGB1 activity.","description":"Megakaryocyte (MK) migration from the bone marrow periosteal niche toward the vascular niche is a prerequisite for proplatelet extension and release into the circulation. The mechanism for this highly coordinated process is poorly understood. Here we show that dynasore (DNSR), a small-molecule inhibitor of dynamins (DNMs), or short hairpin RNA knockdown of DNM2 and DNM3 impairs directional migration in a human MK cell line or MKs derived from cultured CD34<sup>+</sup> cells. Because cell migration requires actin cytoskeletal rearrangements, we measured actin polymerization and the activity of cytoskeleton regulator RhoA and found them to be decreased after inhibition of DNM2 and DNM3. Because SDF-1α is important for hematopoiesis, we studied the expression of its receptor CXCR4 in DNSR-tre","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Dec","modification":"2026-07-16T18:46:14.148Z","creation":"2019-03-27T00:12:24Z"},"accession":"S-EPMC6290100","cross_references":{"pubmed":["30538113"],"doi":["10.1182/bloodadvances.2018021923"]}}