biostudies-literatureDe Roos JVrije Universiteit BrusselKMO PortefeuilleHercules Foundatione02226-18https://www.ebi.ac.uk/biostudies/studies/S-EPMC6293109biostudies-literatureUnknown85(1)Traditional lambic beer production takes place through wort inoculation with environmental air and fermentation and maturation in wooden barrels. These wooden casks or foeders are possible additional inoculation sources of microorganisms for lambic worts. To date, however, these lambic barrels have been examined only with culture-dependent techniques, thereby missing a portion of the microorganisms present. Moreover, the effects of the cleaning procedures (involving high-pressure water and/or fumigation) and the barrel type on the microbial community structures of the interior surfaces of wooden lambic barrels were unclear. The culture-dependent plating and culture-independent amplicon sequencing of swab samples obtained from the interior surfaces of different wooden casks and foeders usedApplied and environmental microbiologyThe Interior Surfaces of Wooden Barrels Are an Additional Microbial Inoculation Source for Lambic Beer Production.PMC6293109SRP72014KMO0849912017KMO1120912015KMO091056UABR09004PhD fellowshipUAB13002IOF3422016KMO149170De Roos JDe Vuyst LVan der Veken DfalseThe Interior Surfaces of Wooden Barrels Are an Additional Microbial Inoculation Source for Lambic Beer Production.Traditional lambic beer production takes place through wort inoculation with environmental air and fermentation and maturation in wooden barrels. These wooden casks or foeders are possible additional inoculation sources of microorganisms for lambic worts. To date, however, these lambic barrels have been examined only with culture-dependent techniques, thereby missing a portion of the microorganisms present. Moreover, the effects of the cleaning procedures (involving high-pressure water and/or fumigation) and the barrel type on the microbial community structures of the interior surfaces of wooden lambic barrels were unclear. The culture-dependent plating and culture-independent amplicon sequencing of swab samples obtained from the interior surfaces of different wooden casks and foeders used2019-01-01T00:00:00Z2019 Jan2025-04-19T16:53:56.29Z2019-07-25T07:11:45ZS-EPMC62931093038976810.1128/AEM.02226-1810.1128/aem.02226-18