biostudies-literatureWu QNational Natural Science Foundation of ChinaNational Cancer InstituteNCI NIH HHS582-594https://www.ebi.ac.uk/biostudies/studies/S-EPMC6294659biostudies-literatureUnknown144(3)Krüppel-like factor 5 (KLF5) both suppresses and promotes tumor growth depending on cellular context. The mechanisms underlying tumor promotion could be targetable for therapy. Although a number of transcriptional targets of KLF5 have been identified and implicated in KLF5-mediated tumor growth, how KLF5 regulates these genes remains to be addressed. Here we performed coimmunoprecipitation (co-IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the TSU-Pr1 bladder cancer cell line, in which KLF5 is shown to promote tumor growth, to identify KLF5-interacting nuclear proteins that are necessary for KLF5's tumor promoting function. LC-MS/MS revealed 122 potential KLF5 binding proteins in the nuclear proteins precipitated by the KLF5 antibody, and the top nine candidates incluInternational journal of cancerCINP is a novel cofactor of KLF5 required for its role in the promotion of cell proliferation, survival and tumor growth.PMC6294659R01 CA171189R01CA17118981130044Gao AQi LLi JAn NLiu MWu QFu XDong JTA JLi MLi YFu CLi ZKing JLFu LZhang BCi XMa GfalseCINP is a novel cofactor of KLF5 required for its role in the promotion of cell proliferation, survival and tumor growth.Krüppel-like factor 5 (KLF5) both suppresses and promotes tumor growth depending on cellular context. The mechanisms underlying tumor promotion could be targetable for therapy. Although a number of transcriptional targets of KLF5 have been identified and implicated in KLF5-mediated tumor growth, how KLF5 regulates these genes remains to be addressed. Here we performed coimmunoprecipitation (co-IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the TSU-Pr1 bladder cancer cell line, in which KLF5 is shown to promote tumor growth, to identify KLF5-interacting nuclear proteins that are necessary for KLF5's tumor promoting function. LC-MS/MS revealed 122 potential KLF5 binding proteins in the nuclear proteins precipitated by the KLF5 antibody, and the top nine candidates inclu2019-01-01T00:00:00Z2019 Feb2026-05-06T01:23:57.31Z2020-10-29T11:01:41ZS-EPMC62946593028997310.1002/ijc.31908