{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Neubrand VE"],"funding":["Michael J. Fox Foundation for Parkinson's Research","Excellence Grant of the Andalusian Government","Michael J. Fox Foundation for Parkinson&apos;s Research"],"pagination":["343"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6295018"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["<h4>Background</h4>Over-activated microglia play a central role during neuroinflammation, leading to neuronal cell death and neurodegeneration. Reversion of over-activated to neuroprotective microglia phenotype could regenerate a healthy CNS-supporting microglia environment. Our aim was to identify a dataset of intracellular molecules in primary microglia that play a role in the transition of microglia to a ramified, neuroprotective phenotype.<h4>Methods</h4>We exploited the anti-inflammatory and neuroprotective properties of conditioned medium of adipose-derived mesenchymal stem cells (CM) as a tool to generate the neuroprotective phenotype of microglia in vitro, and we set up a microscopy-based siRNA screen to identify its hits by cell morphology.<h4>Results</h4>We initially assayed an a"],"journal":["Journal of neuroinflammation"],"pubmed_title":["The atypical RhoGTPase RhoE/Rnd3 is a key molecule to acquire a neuroprotective phenotype in microglia."],"pmcid":["PMC6295018"],"funding_grant_id":["CTS 2939","Grant ID 9404"],"pubmed_authors":["Forte-Lago I","Delgado M","Caro M","Neubrand VE"],"additional_accession":[]},"is_claimable":false,"name":"The atypical RhoGTPase RhoE/Rnd3 is a key molecule to acquire a neuroprotective phenotype in microglia.","description":"<h4>Background</h4>Over-activated microglia play a central role during neuroinflammation, leading to neuronal cell death and neurodegeneration. Reversion of over-activated to neuroprotective microglia phenotype could regenerate a healthy CNS-supporting microglia environment. Our aim was to identify a dataset of intracellular molecules in primary microglia that play a role in the transition of microglia to a ramified, neuroprotective phenotype.<h4>Methods</h4>We exploited the anti-inflammatory and neuroprotective properties of conditioned medium of adipose-derived mesenchymal stem cells (CM) as a tool to generate the neuroprotective phenotype of microglia in vitro, and we set up a microscopy-based siRNA screen to identify its hits by cell morphology.<h4>Results</h4>We initially assayed an a","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Dec","modification":"2026-07-13T03:11:48.898Z","creation":"2019-03-27T00:12:31Z"},"accession":"S-EPMC6295018","cross_references":{"pubmed":["30553270"],"doi":["10.1186/s12974-018-1386-z"]}}