biostudies-literatureUnknown10Morino TThe Jikei University Strategic Research FundFormation of Innovation Center for Fusion of Advanced Technologies<h4>Introduction</h4>Carrier-free autologous mucosal epithelial cell sheets have been clinically utilized as a cell therapy for various epithelial disorders. Fabrication of a transplantable oral mucosal epithelial cell sheet without mouse feeder layers requires a higher seeding density than that of a sheet with mouse feeder layer culture; therefore, a large amount of donor mucosal tissue is needed. However, cell grafts co-cultured with mouse feeder layers are classified by the US Food and Drug Administration (FDA) as xenogeneic products. The goal of this study was to evaluate the utility of oral mucosal epithelial cells expanded by primary explant culture for the fabrication of an adequate number of transplantable epithelial cell sheets without mouse feeder layers.<h4>Methods</h4>Small fraRegenerative therapy36-45https://www.ebi.ac.uk/biostudies/studies/S-EPMC6298907biostudies-literatureExplant culture of oral mucosal epithelial cells for fabricating transplantable epithelial cell sheet.PMC6298907Yamamoto KYamato MKojima HTakagi RMorino TfalseExplant culture of oral mucosal epithelial cells for fabricating transplantable epithelial cell sheet.<h4>Introduction</h4>Carrier-free autologous mucosal epithelial cell sheets have been clinically utilized as a cell therapy for various epithelial disorders. Fabrication of a transplantable oral mucosal epithelial cell sheet without mouse feeder layers requires a higher seeding density than that of a sheet with mouse feeder layer culture; therefore, a large amount of donor mucosal tissue is needed. However, cell grafts co-cultured with mouse feeder layers are classified by the US Food and Drug Administration (FDA) as xenogeneic products. The goal of this study was to evaluate the utility of oral mucosal epithelial cells expanded by primary explant culture for the fabrication of an adequate number of transplantable epithelial cell sheets without mouse feeder layers.<h4>Methods</h4>Small fra2019-01-01T00:00:00Z2019 Jun2026-07-09T12:26:57.97Z2019-03-27T00:13:37ZS-EPMC62989073058189510.1016/j.reth.2018.10.006