biostudies-literatureKim JNICHD NIH HHSNIDDK NIH HHSHoward Hughes Medical InstituteNational Heart, Lung, and Blood InstituteNHLBI NIH HHSNCI NIH HHSNational Institutes of Health39https://www.ebi.ac.uk/biostudies/studies/S-EPMC6309084biostudies-literatureUnknown8(1)<h4>Background</h4>β<sub>2</sub>-adrenergic receptors (β<sub>2</sub>ARs) are the target of catecholamines and play fundamental roles in cardiovascular, pulmonary, and skeletal muscle physiology. An important action of β<sub>2</sub>AR stimulation on skeletal muscle is anabolic growth, which has led to the use of agonists such as clenbuterol by athletes to enhance muscle performance. While previous work has demonstrated that β<sub>2</sub>ARs can engage distinct signaling and functional cascades mediated by either G proteins or the multifunctional adaptor protein, β-arrestin, the precise role of β-arrestin in skeletal muscle physiology is not known. Here, we tested the hypothesis that agonist activation of the β<sub>2</sub>AR by clenbuterol would engage β-arrestin as a key transducer of anaboSkeletal muscleβ-arrestin 1 regulates β2-adrenergic receptor-mediated skeletal muscle hypertrophy and contractility.PMC6309084P01 HL075443HD070872R01-CA172570K08 HL133488R01 CA172570K12 CA100639HL056687HL133488R01 HL056687HL16037R01 DK109911R01 HL016037DK1099115K12-CA100639-08K08 HD070872NoneHL075443Li TChen MChen WGrotegut CARosenberg PBKim JLefkowitz RJWisler JWMao LRockman HAfalseβ-arrestin 1 regulates β2-adrenergic receptor-mediated skeletal muscle hypertrophy and contractility.<h4>Background</h4>β<sub>2</sub>-adrenergic receptors (β<sub>2</sub>ARs) are the target of catecholamines and play fundamental roles in cardiovascular, pulmonary, and skeletal muscle physiology. An important action of β<sub>2</sub>AR stimulation on skeletal muscle is anabolic growth, which has led to the use of agonists such as clenbuterol by athletes to enhance muscle performance. While previous work has demonstrated that β<sub>2</sub>ARs can engage distinct signaling and functional cascades mediated by either G proteins or the multifunctional adaptor protein, β-arrestin, the precise role of β-arrestin in skeletal muscle physiology is not known. Here, we tested the hypothesis that agonist activation of the β<sub>2</sub>AR by clenbuterol would engage β-arrestin as a key transducer of anabo2018-01-01T00:00:00Z2018 Dec2025-04-21T21:33:47.838Z2019-03-26T22:33:50ZS-EPMC63090843059107910.1186/s13395-018-0184-8