{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(1)"],"submitter":["Son D"],"pubmed_abstract":["Lymphocyte antigen 6 family member K (LY6K) is upregulated in a number of types of cancer and promotes tumor cell proliferation and metastasis. In addition, LY6K is involved in tamoxifen resistance in breast cancer. However, the <i>in vivo</i> molecular mechanism of LY6K has not yet been investigated. In the present study, transgenic mice overexpressing human LY6K (hLY6K) were generated using the pMAMneo vector, and the effect of LY6K upregulation <i>in vivo</i> was investigated. A total of 4 transgenic mice were generated, and the gene copy number was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR demonstrated that mRNA of hLY6K was overexpressed in the thymus and spleen of the transgenic mice compared with wild-type mice. Flow cytometric an"],"journal":["Oncology letters"],"pagination":["379-387"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6313091"],"repository":["biostudies-literature"],"pubmed_title":["Transgenic overexpression of human <i>LY6K</i> in mice suppresses mature T cell development in the thymus."],"pmcid":["PMC6313091"],"pubmed_authors":["Son D","Song MJ","Park JH","Kim Y","Kong HK","Kim HP","Lee HW"],"additional_accession":[]},"is_claimable":false,"name":"Transgenic overexpression of human <i>LY6K</i> in mice suppresses mature T cell development in the thymus.","description":"Lymphocyte antigen 6 family member K (LY6K) is upregulated in a number of types of cancer and promotes tumor cell proliferation and metastasis. In addition, LY6K is involved in tamoxifen resistance in breast cancer. However, the <i>in vivo</i> molecular mechanism of LY6K has not yet been investigated. In the present study, transgenic mice overexpressing human LY6K (hLY6K) were generated using the pMAMneo vector, and the effect of LY6K upregulation <i>in vivo</i> was investigated. A total of 4 transgenic mice were generated, and the gene copy number was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR demonstrated that mRNA of hLY6K was overexpressed in the thymus and spleen of the transgenic mice compared with wild-type mice. Flow cytometric an","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2025-04-04T10:03:36.019Z","creation":"2019-03-26T22:37:32Z"},"accession":"S-EPMC6313091","cross_references":{"pubmed":["30655778"],"doi":["10.3892/ol.2018.9548"]}}