{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["de Lazaro I"],"funding":["Royal College of Surgeons of Edinburgh","Obra Social LaCaixa","UCL School of Pharmacy"],"pagination":["59-75"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6318817"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(1)"],"pubmed_abstract":["Overexpression of Oct3/4, Klf4, Sox2, and c-Myc (OKSM) transcription factors can de-differentiate adult cells in vivo. While sustained OKSM expression triggers tumorigenesis through uncontrolled proliferation of toti- and pluripotent cells, transient reprogramming induces pluripotency-like features and proliferation only temporarily, without teratomas. We sought to transiently reprogram cells within mouse skeletal muscle with a localized injection of plasmid DNA encoding OKSM (pOKSM), and we hypothesized that the generation of proliferative intermediates would enhance tissue regeneration after injury. Intramuscular pOKSM administration rapidly upregulated pluripotency (Nanog, Ecat1, and Rex1) and early myogenesis genes (Pax3) in the healthy gastrocnemius of various strains. Mononucleated c"],"journal":["Molecular therapy : the journal of the American Society of Gene Therapy"],"pubmed_title":["Non-viral, Tumor-free Induction of Transient Cell Reprogramming in Mouse Skeletal Muscle to Enhance Tissue Regeneration."],"pmcid":["PMC6318817"],"funding_grant_id":["KAE WONJ4"],"pubmed_authors":["Razak FMA","Kostarelos K","Yilmazer A","Qubisi S","Cossu G","Degens H","Nam Y","de Lazaro I"],"additional_accession":[]},"is_claimable":false,"name":"Non-viral, Tumor-free Induction of Transient Cell Reprogramming in Mouse Skeletal Muscle to Enhance Tissue Regeneration.","description":"Overexpression of Oct3/4, Klf4, Sox2, and c-Myc (OKSM) transcription factors can de-differentiate adult cells in vivo. While sustained OKSM expression triggers tumorigenesis through uncontrolled proliferation of toti- and pluripotent cells, transient reprogramming induces pluripotency-like features and proliferation only temporarily, without teratomas. We sought to transiently reprogram cells within mouse skeletal muscle with a localized injection of plasmid DNA encoding OKSM (pOKSM), and we hypothesized that the generation of proliferative intermediates would enhance tissue regeneration after injury. Intramuscular pOKSM administration rapidly upregulated pluripotency (Nanog, Ecat1, and Rex1) and early myogenesis genes (Pax3) in the healthy gastrocnemius of various strains. Mononucleated c","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2025-04-22T07:39:38.032Z","creation":"2025-04-05T22:11:11.795Z"},"accession":"S-EPMC6318817","cross_references":{"pubmed":["30470628"],"doi":["10.1016/j.ymthe.2018.10.014"]}}