{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xin H"],"funding":["National Natural Science Foundation of China"],"pagination":["30"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6325824"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["<h4>Background</h4>MicroRNA-196a-5p (miR-196a-5p) has been reported to be involved in the metastatic process of several cancers. In present work, we aimed to investigate the effects of miR-196a-5p and its potential target IκBα on migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells.<h4>Methods</h4>CCK-8 assay, wound healing assay and cell invasion assay were performed to evaluate the cell proliferation, migration and invasion. In vivo metastasis models were used to investigate the tumor metastasis ability. Real-time PCR, immunofluorescence staining or western blot were utilized to detect the expression of miR-196a-5p, IκBα, p-IκBα, nuclear p65 and EMT markers including E-cadherin, N-cadherin and fibronectin. Dual luciferase reporter assay was car"],"journal":["BMC cancer"],"pubmed_title":["miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα."],"pmcid":["PMC6325824"],"funding_grant_id":["81470086"],"pubmed_authors":["Liu Z","Wang C","Xin H"],"additional_accession":[]},"is_claimable":false,"name":"miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα.","description":"<h4>Background</h4>MicroRNA-196a-5p (miR-196a-5p) has been reported to be involved in the metastatic process of several cancers. In present work, we aimed to investigate the effects of miR-196a-5p and its potential target IκBα on migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells.<h4>Methods</h4>CCK-8 assay, wound healing assay and cell invasion assay were performed to evaluate the cell proliferation, migration and invasion. In vivo metastasis models were used to investigate the tumor metastasis ability. Real-time PCR, immunofluorescence staining or western blot were utilized to detect the expression of miR-196a-5p, IκBα, p-IκBα, nuclear p65 and EMT markers including E-cadherin, N-cadherin and fibronectin. Dual luciferase reporter assay was car","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2025-04-04T18:56:15.927Z","creation":"2019-03-26T22:36:25Z"},"accession":"S-EPMC6325824","cross_references":{"pubmed":["30621631"],"doi":["10.1186/s12885-018-5245-1"]}}