biostudies-literatureXin HNational Natural Science Foundation of China30https://www.ebi.ac.uk/biostudies/studies/S-EPMC6325824biostudies-literatureUnknown19(1)<h4>Background</h4>MicroRNA-196a-5p (miR-196a-5p) has been reported to be involved in the metastatic process of several cancers. In present work, we aimed to investigate the effects of miR-196a-5p and its potential target IκBα on migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells.<h4>Methods</h4>CCK-8 assay, wound healing assay and cell invasion assay were performed to evaluate the cell proliferation, migration and invasion. In vivo metastasis models were used to investigate the tumor metastasis ability. Real-time PCR, immunofluorescence staining or western blot were utilized to detect the expression of miR-196a-5p, IκBα, p-IκBα, nuclear p65 and EMT markers including E-cadherin, N-cadherin and fibronectin. Dual luciferase reporter assay was carBMC cancermiR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα.PMC632582481470086Liu ZWang CXin HfalsemiR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα.<h4>Background</h4>MicroRNA-196a-5p (miR-196a-5p) has been reported to be involved in the metastatic process of several cancers. In present work, we aimed to investigate the effects of miR-196a-5p and its potential target IκBα on migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells.<h4>Methods</h4>CCK-8 assay, wound healing assay and cell invasion assay were performed to evaluate the cell proliferation, migration and invasion. In vivo metastasis models were used to investigate the tumor metastasis ability. Real-time PCR, immunofluorescence staining or western blot were utilized to detect the expression of miR-196a-5p, IκBα, p-IκBα, nuclear p65 and EMT markers including E-cadherin, N-cadherin and fibronectin. Dual luciferase reporter assay was car2019-01-01T00:00:00Z2019 Jan2025-04-04T18:56:15.927Z2019-03-26T22:36:25ZS-EPMC63258243062163110.1186/s12885-018-5245-1