biostudies-literatureUnknown10(1)Hu PAcidosis is a significant feature of the tumor microenvironment in glioma, and it is closely related to multiple biological functions of cancer stem cells. Here, we found that the self-renewal ability, the mitochondrial activity and ATP production were elevated in stem cell-like glioma cells (SLCs) under acidic microenvironment, which promoted and maintained the stemness of SLCs. Under acidosis, 25-hydroxy vitamin D<sub>3</sub>-24-hydroxylase (CYP24A1) was upregulated and catalyzed the fast degradation of 1α,25(OH)<sub>2</sub>D<sub>3</sub>. We further revealed that the active form of vitamin D (1α,25(OH)<sub>2</sub>D<sub>3</sub>) could inhibit the expression of stemness markers, attenuate acidosis-induced increase of self-renewal ability and mitochondrial respiration in stem cell-like glioCell death & disease25https://www.ebi.ac.uk/biostudies/studies/S-EPMC6328565biostudies-literatureAcidosis enhances the self-renewal and mitochondrial respiration of stem cell-like glioma cells through CYP24A1-mediated reduction of vitamin D.PMC6328565Peng XHu YLi DHan WJiang TWei ZWu FYuan JLi YLi SQiang BWei QQi YYin BTian NHu PfalseAcidosis enhances the self-renewal and mitochondrial respiration of stem cell-like glioma cells through CYP24A1-mediated reduction of vitamin D.Acidosis is a significant feature of the tumor microenvironment in glioma, and it is closely related to multiple biological functions of cancer stem cells. Here, we found that the self-renewal ability, the mitochondrial activity and ATP production were elevated in stem cell-like glioma cells (SLCs) under acidic microenvironment, which promoted and maintained the stemness of SLCs. Under acidosis, 25-hydroxy vitamin D<sub>3</sub>-24-hydroxylase (CYP24A1) was upregulated and catalyzed the fast degradation of 1α,25(OH)<sub>2</sub>D<sub>3</sub>. We further revealed that the active form of vitamin D (1α,25(OH)<sub>2</sub>D<sub>3</sub>) could inhibit the expression of stemness markers, attenuate acidosis-induced increase of self-renewal ability and mitochondrial respiration in stem cell-like glio2019-01-01T00:00:00Z2019 Jan2025-04-04T10:13:28.19Z2019-03-26T22:36:28ZS-EPMC63285653063103510.1038/s41419-018-1242-1