{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(1)"],"submitter":["Skottvoll FS"],"funding":["Research Council of Norway through its Centres of Excellence scheme"],"pubmed_abstract":["<h4>Aim</h4>For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared.<h4>Materials & methods</h4>'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed.<h4>Results</h4>Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC-MS. Western blotting and nanoLC-MS also revealed negative exosome markers regarding both isolation approaches.<h4>Conclusion</h4>The two isolation methods had an overall similar performance, but we hesitate to use the term 'exosome isolation' as impurities may be present with"],"journal":["Future science OA"],"pagination":["FSO359"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6331754"],"repository":["biostudies-literature"],"pubmed_title":["Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations."],"pmcid":["PMC6331754"],"pubmed_authors":["Roberg-Larsen H","Skottvoll FS","Thiede B","Nystrom B","Bekhradnia S","Bjorseth K","Wilson SR","Berg HE","Roos N","Sandberg C","Lundanes E","Lund K","Vik-Mo EO"],"additional_accession":[]},"is_claimable":false,"name":"Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations.","description":"<h4>Aim</h4>For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared.<h4>Materials & methods</h4>'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed.<h4>Results</h4>Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC-MS. Western blotting and nanoLC-MS also revealed negative exosome markers regarding both isolation approaches.<h4>Conclusion</h4>The two isolation methods had an overall similar performance, but we hesitate to use the term 'exosome isolation' as impurities may be present with","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2026-05-06T20:24:47.656Z","creation":"2019-03-26T22:38:01Z"},"accession":"S-EPMC6331754","cross_references":{"pubmed":["30652024"],"doi":["10.4155/fsoa-2018-0088"]}}