biostudies-literatureUnknown5(1)Skottvoll FSResearch Council of Norway through its Centres of Excellence scheme<h4>Aim</h4>For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared.<h4>Materials & methods</h4>'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed.<h4>Results</h4>Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC-MS. Western blotting and nanoLC-MS also revealed negative exosome markers regarding both isolation approaches.<h4>Conclusion</h4>The two isolation methods had an overall similar performance, but we hesitate to use the term 'exosome isolation' as impurities may be present withFuture science OAFSO359https://www.ebi.ac.uk/biostudies/studies/S-EPMC6331754biostudies-literatureUltracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations.PMC6331754Roberg-Larsen HSkottvoll FSThiede BNystrom BBekhradnia SBjorseth KWilson SRBerg HERoos NSandberg CLundanes ELund KVik-Mo EOfalseUltracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations.<h4>Aim</h4>For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared.<h4>Materials & methods</h4>'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed.<h4>Results</h4>Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC-MS. Western blotting and nanoLC-MS also revealed negative exosome markers regarding both isolation approaches.<h4>Conclusion</h4>The two isolation methods had an overall similar performance, but we hesitate to use the term 'exosome isolation' as impurities may be present with2019-01-01T00:00:00Z2019 Jan2026-05-06T20:24:47.656Z2019-03-26T22:38:01ZS-EPMC63317543065202410.4155/fsoa-2018-0088