biostudies-literatureUnknown9Sanahuja ITemperate fish are particularly sensitive to low temperatures, especially in the northern Mediterranean area, where the cold season decreases fish-farm production and affects fish health. Recent studies have suggested that the skin mucus participates in overall fish defense and welfare, and therefore propose it as a target for non-invasive studies of fish status. Here, we determine the mucus interactome of differentially expressed proteins in a temperate fish model, gilthead sea bream (<i>Sparus aurata</i>), after chronic exposure to low temperatures (7 weeks at 14°C). The differentially expressed proteins were obtained by 2D-PAGE of mucus soluble proteins and further assessed by STRING analyses of the functional interactome based on protein-protein interactions. Complementarily, we determFrontiers in physiology1916https://www.ebi.ac.uk/biostudies/studies/S-EPMC6336924biostudies-literatureChronic Cold Stress Alters the Skin Mucus Interactome in a Temperate Fish Model.PMC6336924Sanchez-Nuno SFernandez-Alacid LSanahuja IOrdonez-Grande BIbarz AfalseChronic Cold Stress Alters the Skin Mucus Interactome in a Temperate Fish Model.Temperate fish are particularly sensitive to low temperatures, especially in the northern Mediterranean area, where the cold season decreases fish-farm production and affects fish health. Recent studies have suggested that the skin mucus participates in overall fish defense and welfare, and therefore propose it as a target for non-invasive studies of fish status. Here, we determine the mucus interactome of differentially expressed proteins in a temperate fish model, gilthead sea bream (<i>Sparus aurata</i>), after chronic exposure to low temperatures (7 weeks at 14°C). The differentially expressed proteins were obtained by 2D-PAGE of mucus soluble proteins and further assessed by STRING analyses of the functional interactome based on protein-protein interactions. Complementarily, we determ2018-01-01T00:00:00Z20182026-04-08T01:54:29.522Z2019-03-26T22:43:42ZS-EPMC63369243068712610.3389/fphys.2018.01916