biostudies-literatureSu Jgrants from the Infective Diseases Prevention and Cure Project of National Ministry of Public Health of China44https://www.ebi.ac.uk/biostudies/studies/S-EPMC6339288biostudies-literatureUnknown12(1)<h4>Background</h4>The mosquito Aedes albopictus is an important vector for dengue virus (DENV) transmission. The midgut is the first barrier to mosquito infection by DENV, and this barrier is a critical factor affecting the vector competence of the mosquito. However, the molecular mechanism of the interaction between midgut and virus is unknown.<h4>Results</h4>Six small libraries of Ae. albopictus midgut RNAs were constructed, three of which from mosquitoes that were infected with DENV-2 after feeding on infected blood, and another three that remained uninfected with DENV-2 after feeding on same batch of infected blood. A total of 46 differentially expressed miRNAs were identified of which 17 significant differentially expressed miRNAs were selected. Compared to microRNA expression profilParasites & vectorsScreening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV-2 and their effect on replication of DENV-2 in C6/36 cells.PMC63392882012ZX10004219Li CWu QGuo XYan TLiu QWang GZhu XSu JXing DZhao TfalseScreening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV-2 and their effect on replication of DENV-2 in C6/36 cells.<h4>Background</h4>The mosquito Aedes albopictus is an important vector for dengue virus (DENV) transmission. The midgut is the first barrier to mosquito infection by DENV, and this barrier is a critical factor affecting the vector competence of the mosquito. However, the molecular mechanism of the interaction between midgut and virus is unknown.<h4>Results</h4>Six small libraries of Ae. albopictus midgut RNAs were constructed, three of which from mosquitoes that were infected with DENV-2 after feeding on infected blood, and another three that remained uninfected with DENV-2 after feeding on same batch of infected blood. A total of 46 differentially expressed miRNAs were identified of which 17 significant differentially expressed miRNAs were selected. Compared to microRNA expression profil2019-01-01T00:00:00Z2019 Jan2026-05-03T01:59:32.434Z2025-05-29T21:46:38.982ZS-EPMC63392883065869210.1186/s13071-018-3261-2