biostudies-literatureRamasamy MTexas A&M AgriLifeNational Institute of Food and Agriculture211-227https://www.ebi.ac.uk/biostudies/studies/S-EPMC6343536biostudies-literatureUnknown9(4)Sugarcane and energycane (Saccharum spp. hybrids) are prominent sources of sugar, ethanol, as well as high-value bioproducts globally. Genetic analysis for trait improvement of sugarcane is greatly hindered by its complex genome, limited germplasm resources, long breeding cycle, as well as recalcitrance to genetic transformation. Here, we present a biolistic-based transformation and bioreactor-based micro-propagation system that has been utilized successfully to transform twelve elite cane genotypes, yielding transformation efficiencies of up to 39%. The system relies on the generation of embryogenic callus from sugarcane and energycane apical shoot tissue, followed by DNA bombardment of embryogenic leaf roll discs (approximately one week) or calli (approximately 4 weeks). We present optimGM crops & foodA biolistic-based genetic transformation system applicable to a broad-range of sugarcane and energycane varieties.PMC6343536124738-92810124738-96210HATCH TEX09621Mora VRamasamy MRossi DDaSilva JAMirkov TESolis-Gracia NIrigoyen SDamaj MBRamos NMandadi KKPadilla CSVargas-Bautista CfalseA biolistic-based genetic transformation system applicable to a broad-range of sugarcane and energycane varieties.Sugarcane and energycane (Saccharum spp. hybrids) are prominent sources of sugar, ethanol, as well as high-value bioproducts globally. Genetic analysis for trait improvement of sugarcane is greatly hindered by its complex genome, limited germplasm resources, long breeding cycle, as well as recalcitrance to genetic transformation. Here, we present a biolistic-based transformation and bioreactor-based micro-propagation system that has been utilized successfully to transform twelve elite cane genotypes, yielding transformation efficiencies of up to 39%. The system relies on the generation of embryogenic callus from sugarcane and energycane apical shoot tissue, followed by DNA bombardment of embryogenic leaf roll discs (approximately one week) or calli (approximately 4 weeks). We present optim2018-01-01T00:00:00Z20182025-04-04T18:56:17.854Z2020-05-21T23:04:41ZS-EPMC63435363055847210.1080/21645698.2018.1553836