{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kumar A"],"funding":["NCRR NIH HHS","NHLBI NIH HHS","NIH HHS"],"pagination":["770-776"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6344284"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["202(3)"],"pubmed_abstract":["Human pluripotent stem cells (hPSCs) offer the potential to serve as a versatile and scalable source of T cells for immunotherapies, which could be coupled with genetic engineering technologies to meet specific clinical needs. To improve T cell production from hPSCs, it is essential to identify cell subsets that are highly enriched in T cell progenitors and those stages of development at which NOTCH activation induces the most potent T cells. In this study, we evaluated the efficacy of T cell production from cell populations isolated at different stages of hematopoietic differentiation, including mesoderm, hemogenic endothelium (HE), and multipotent hematopoietic progenitors. We demonstrate that KDR<sup>hi</sup>CD31<sup>-</sup> hematovascular mesodermal progenitors (HVMPs) with definitive "],"journal":["Journal of immunology (Baltimore, Md. : 1950)"],"pubmed_title":["NOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells."],"pmcid":["PMC6344284"],"funding_grant_id":["R01 HL142665","P51 RR000167","P51 OD011106"],"pubmed_authors":["Thakur AS","Suknuntha K","D'Souza SS","Slukvin II","Kumar A","Lee JH"],"additional_accession":[]},"is_claimable":false,"name":"NOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells.","description":"Human pluripotent stem cells (hPSCs) offer the potential to serve as a versatile and scalable source of T cells for immunotherapies, which could be coupled with genetic engineering technologies to meet specific clinical needs. To improve T cell production from hPSCs, it is essential to identify cell subsets that are highly enriched in T cell progenitors and those stages of development at which NOTCH activation induces the most potent T cells. In this study, we evaluated the efficacy of T cell production from cell populations isolated at different stages of hematopoietic differentiation, including mesoderm, hemogenic endothelium (HE), and multipotent hematopoietic progenitors. We demonstrate that KDR<sup>hi</sup>CD31<sup>-</sup> hematovascular mesodermal progenitors (HVMPs) with definitive ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Feb","modification":"2025-04-21T15:09:12.853Z","creation":"2020-10-29T11:00:00Z"},"accession":"S-EPMC6344284","cross_references":{"pubmed":["30578305"],"doi":["10.4049/jimmunol.1801027"]}}