biostudies-literatureKumar ANCRR NIH HHSNHLBI NIH HHSNIH HHS770-776https://www.ebi.ac.uk/biostudies/studies/S-EPMC6344284biostudies-literatureUnknown202(3)Human pluripotent stem cells (hPSCs) offer the potential to serve as a versatile and scalable source of T cells for immunotherapies, which could be coupled with genetic engineering technologies to meet specific clinical needs. To improve T cell production from hPSCs, it is essential to identify cell subsets that are highly enriched in T cell progenitors and those stages of development at which NOTCH activation induces the most potent T cells. In this study, we evaluated the efficacy of T cell production from cell populations isolated at different stages of hematopoietic differentiation, including mesoderm, hemogenic endothelium (HE), and multipotent hematopoietic progenitors. We demonstrate that KDR^hiCD31^- hematovascular mesodermal progenitors (HVMPs) with definitive Journal of immunology (Baltimore, Md. : 1950)NOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells.PMC6344284R01 HL142665P51 RR000167P51 OD011106Thakur ASSuknuntha KD'Souza SSSlukvin IIKumar ALee JHfalseNOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells.Human pluripotent stem cells (hPSCs) offer the potential to serve as a versatile and scalable source of T cells for immunotherapies, which could be coupled with genetic engineering technologies to meet specific clinical needs. To improve T cell production from hPSCs, it is essential to identify cell subsets that are highly enriched in T cell progenitors and those stages of development at which NOTCH activation induces the most potent T cells. In this study, we evaluated the efficacy of T cell production from cell populations isolated at different stages of hematopoietic differentiation, including mesoderm, hemogenic endothelium (HE), and multipotent hematopoietic progenitors. We demonstrate that KDR^hiCD31^- hematovascular mesodermal progenitors (HVMPs) with definitive 2019-01-01T00:00:00Z2019 Feb2025-04-21T15:09:12.853Z2020-10-29T11:00:00ZS-EPMC63442843057830510.4049/jimmunol.1801027