biostudies-literatureFreund EBundesministerium für Bildung und Forschung634https://www.ebi.ac.uk/biostudies/studies/S-EPMC6345938biostudies-literatureUnknown9(1)Metastatic colorectal cancer is the fourth most common cause of cancer death. Current options in palliation such as hyperthermic intraperitoneal chemotherapy (HIPEC) present severe side effects. Recent research efforts suggested the therapeutic use of oxidant-enriched liquid using cold physical plasma. To investigate a clinically accepted treatment regimen, we assessed the antitumor capacity of plasma-treated saline solution. In response to such liquid, CT26 murine colon cancer cells were readily oxidized and showed cell growth with subsequent apoptosis, cell cycle arrest, and upregulation of immunogenic cell death (ICD) markers in vitro. This was accompanied by marked morphological changes with re-arrangement of actin fibers and reduced motility. Induction of an epithelial-to-mesenchymal Scientific reportsPhysical plasma-treated saline promotes an immunogenic phenotype in CT26 colon cancer cells in vitro and in vivo.PMC634593803Z22DN11Freund EBekeschus SPartecke LIHeidecke CDLiedtke KRMetelmann HRvan der Linde JfalsePhysical plasma-treated saline promotes an immunogenic phenotype in CT26 colon cancer cells in vitro and in vivo.Metastatic colorectal cancer is the fourth most common cause of cancer death. Current options in palliation such as hyperthermic intraperitoneal chemotherapy (HIPEC) present severe side effects. Recent research efforts suggested the therapeutic use of oxidant-enriched liquid using cold physical plasma. To investigate a clinically accepted treatment regimen, we assessed the antitumor capacity of plasma-treated saline solution. In response to such liquid, CT26 murine colon cancer cells were readily oxidized and showed cell growth with subsequent apoptosis, cell cycle arrest, and upregulation of immunogenic cell death (ICD) markers in vitro. This was accompanied by marked morphological changes with re-arrangement of actin fibers and reduced motility. Induction of an epithelial-to-mesenchymal 2019-01-01T00:00:00Z2019 Jan2025-04-26T08:51:20.636Z2019-03-26T22:41:53ZS-EPMC63459383067972010.1038/s41598-018-37169-3