biostudies-literatureKageyama SNIDDK NIH HHSNIAID NIH HHSNIEHS NIH HHSNCI NIH HHSNational Institutes of HealthCSR NIH HHSCenter for Scientific Review356-367https://www.ebi.ac.uk/biostudies/studies/S-EPMC6349504biostudies-literatureUnknown19(2)By documenting potent antioxidative and anti-inflammatory functions, preclinical studies encourage heme oxygenase-1 (HO-1)-inducing regimens in clinical orthotopic liver transplantation (OLT). We aimed to determine the importance of recipient-derived HO-1 in murine and human OLTs. Hepatic biopsies from 51 OLT patients were screened for HO-1 expression (Western blots) prior to put-in (basal) and post reperfusion (stressed) and correlated with the hepatocellular function. In parallel, livers from HO-1 proficient mice (WT; C57/BL6), subjected to ex vivo cold storage (18 hour), were transplanted to syngeneic myeloid HO-1 deficient (mHO-1 KO) or FLOX (control) hosts, and sampled postreperfusion (6 hour). In human OLT, posttransplant but not pretransplant HO-1 expression correlated negatively wiAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsRecipient HO-1 inducibility is essential for posttransplant hepatic HO-1 expression and graft protection: From bench-to-bedside.PMC6349504RO1 ES016959P01 AI120944T32 CA009120R01 ES016959RO1 DK062357R01 DK102110R01 DK107533R56 ES016959R56 ES016959-06DK102110DK107533PO1 AI120944R01 DK062357Busuttil RWIto TNakamura KHirao HKageyama SKaldas FMZhang MOncel DSosa RAAziz AKupiec-Weglinski JWReed EFKe BAraujo JAfalseRecipient HO-1 inducibility is essential for posttransplant hepatic HO-1 expression and graft protection: From bench-to-bedside.By documenting potent antioxidative and anti-inflammatory functions, preclinical studies encourage heme oxygenase-1 (HO-1)-inducing regimens in clinical orthotopic liver transplantation (OLT). We aimed to determine the importance of recipient-derived HO-1 in murine and human OLTs. Hepatic biopsies from 51 OLT patients were screened for HO-1 expression (Western blots) prior to put-in (basal) and post reperfusion (stressed) and correlated with the hepatocellular function. In parallel, livers from HO-1 proficient mice (WT; C57/BL6), subjected to ex vivo cold storage (18 hour), were transplanted to syngeneic myeloid HO-1 deficient (mHO-1 KO) or FLOX (control) hosts, and sampled postreperfusion (6 hour). In human OLT, posttransplant but not pretransplant HO-1 expression correlated negatively wi2019-01-01T00:00:00Z2019 Feb2025-04-26T10:41:11.92Z2020-05-22T08:45:33ZS-EPMC63495043005919510.1111/ajt.15043