biostudies-literatureLiao MZNCATS NIH HHSNICHD NIH HHSNIDA NIH HHS145-152https://www.ebi.ac.uk/biostudies/studies/S-EPMC6350206biostudies-literatureUnknown12(2)<h4>Background</h4>Few studies have systematically investigated pregnancy-induced changes in protein abundance of drug transporters in organs important for drug/xenobiotic disposition.<h4>Objective</h4>The goal of this study was to compare protein abundance of important drug/xenobiotic transporters including Abcb1a, Abcg2, Abcc2, and Slco1b2 in the liver, kidney and brain of pregnant mice on gestation day 15 to that of non-pregnant mice.<h4>Methods</h4>The mass spectrometry-based proteomics was used to quantify changes in protein abundance of transporters in tissues from pregnant and non-pregnant mice.<h4>Results</h4>The protein levels of hepatic Abcc2, Abcc3, and Slco1a4 per μg of total membrane proteins were significantly decreased by pregnancy by 24%, 72%, and 70%, respectively. The proDrug metabolism lettersQuantitative Proteomics Reveals Changes in Transporter Protein Abundance in Liver, Kidney and Brain of Mice by Pregnancy.PMC6350206R01 HD081299TL1 TR000422P01 DA032507Liao MZPrasad BMao QGao CBhatt DKfalseQuantitative Proteomics Reveals Changes in Transporter Protein Abundance in Liver, Kidney and Brain of Mice by Pregnancy.<h4>Background</h4>Few studies have systematically investigated pregnancy-induced changes in protein abundance of drug transporters in organs important for drug/xenobiotic disposition.<h4>Objective</h4>The goal of this study was to compare protein abundance of important drug/xenobiotic transporters including Abcb1a, Abcg2, Abcc2, and Slco1b2 in the liver, kidney and brain of pregnant mice on gestation day 15 to that of non-pregnant mice.<h4>Methods</h4>The mass spectrometry-based proteomics was used to quantify changes in protein abundance of transporters in tissues from pregnant and non-pregnant mice.<h4>Results</h4>The protein levels of hepatic Abcc2, Abcc3, and Slco1a4 per μg of total membrane proteins were significantly decreased by pregnancy by 24%, 72%, and 70%, respectively. The pro2018-01-01T00:00:00Z20182026-07-16T20:46:03.173Z2026-07-10T03:12:04.866ZS-EPMC63502062993862310.2174/1872312812666180625122810