biostudies-literatureZhang LNational Natural Science Foundation of ChinaScientific Research Special Funds of Provincial Colleges and UniversitiesFoundation for Key Teacher by Henan University of TechnologyProgram for Science and Technology Innovation Talents in Universities of Henan Province391-406https://www.ebi.ac.uk/biostudies/studies/S-EPMC6351124biostudies-literatureUnknown15(3)Currently, particular focus is placed on the implication of autophagy in a variety of human diseases, including cancer. Discovery of small-molecule modulators of autophagy as well as their potential use as anti-cancer therapeutic agents would be of great significance. To this end, a series of curcumin analogs previously synthesized in our laboratory were screened. Among these compounds, (3E,5E)-3-(3,4-dimethoxybenzylidene)-5-[(1H-indol-3-yl)methylene]-1-methylpiperidin-4-one (CA-5f) was identified as a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion. We found that CA-5f neither impaired the hydrolytic function nor the quantity of lysosomes. Use of an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic screen in combiAutophagyIdentification of compound CA-5f as a novel late-stage autophagy inhibitor with potent anti-tumor effect against non-small cell lung cancer.PMC6351124no. 001170no. 15HASTIT031no. 31471296no. 2015RCJH04Jing HQiang PCui LQu JZhang LYu JYao XLiu YChen ZZhao QMiao YLiu BSun GfalseIdentification of compound CA-5f as a novel late-stage autophagy inhibitor with potent anti-tumor effect against non-small cell lung cancer.Currently, particular focus is placed on the implication of autophagy in a variety of human diseases, including cancer. Discovery of small-molecule modulators of autophagy as well as their potential use as anti-cancer therapeutic agents would be of great significance. To this end, a series of curcumin analogs previously synthesized in our laboratory were screened. Among these compounds, (3E,5E)-3-(3,4-dimethoxybenzylidene)-5-[(1H-indol-3-yl)methylene]-1-methylpiperidin-4-one (CA-5f) was identified as a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion. We found that CA-5f neither impaired the hydrolytic function nor the quantity of lysosomes. Use of an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic screen in combi2019-01-01T00:00:00Z2019 Mar2026-04-07T14:35:33.257Z2019-09-12T07:00:57ZS-EPMC63511243014592510.1080/15548627.2018.1511503