{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(1)"],"submitter":["Mo ZQ"],"pubmed_abstract":["Immobilization antigens (i-antigens) are surface membrane proteins that are widely recognized to be the ideal candidates as vaccines antigens for immunization against Cryptocaryon irritans. In this study, we cloned a putative i-antigen gene from C. irritans, which was expressed in all three stages of the C. irritans life-cycle, and localized primarily to the cell surface. The recombinant GDCI3 i-antigen was expressed and purified using the free-living ciliate, Tetrahymena thermophila as an expression system. The purified recombinant protein was recognized by rabbit anti-C. irritans antiserum and was capable of eliciting immobilizing antibodies in rabbits and fish suggesting that the antigen itself was correctly folded. Following immunization and parasite challenge, groupers vaccinated with"],"journal":["Scientific reports"],"pagination":["1029"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6355922"],"repository":["biostudies-literature"],"pubmed_title":["Characterization and immune regulation role of an immobilization antigen from Cryptocaryon irritans on groupers."],"pmcid":["PMC6355922"],"pubmed_authors":["Cassidy-Hanley DM","Mo ZQ","Li AX","Dan XM","Xu S","Li YW","Kolbin D","Fricke JM","Clark TG"],"additional_accession":[]},"is_claimable":false,"name":"Characterization and immune regulation role of an immobilization antigen from Cryptocaryon irritans on groupers.","description":"Immobilization antigens (i-antigens) are surface membrane proteins that are widely recognized to be the ideal candidates as vaccines antigens for immunization against Cryptocaryon irritans. In this study, we cloned a putative i-antigen gene from C. irritans, which was expressed in all three stages of the C. irritans life-cycle, and localized primarily to the cell surface. The recombinant GDCI3 i-antigen was expressed and purified using the free-living ciliate, Tetrahymena thermophila as an expression system. The purified recombinant protein was recognized by rabbit anti-C. irritans antiserum and was capable of eliciting immobilizing antibodies in rabbits and fish suggesting that the antigen itself was correctly folded. Following immunization and parasite challenge, groupers vaccinated with","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2026-05-03T17:03:34.754Z","creation":"2019-03-26T22:50:36Z"},"accession":"S-EPMC6355922","cross_references":{"pubmed":["30705292"],"doi":["10.1038/s41598-018-25710-3"]}}