{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Khayrullin A"],"funding":["National Institute on Aging"],"pagination":["E37"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6356348"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Extracellular vesicles (EVs), including exosomes and microvesicles, function in cell-to-cell communication through delivery of proteins, lipids and microRNAs to target cells via endocytosis and membrane fusion. These vesicles are enriched in ceramide, a sphingolipid associated with the promotion of cell senescence and apoptosis. We investigated the ceramide profile of serum exosomes from young (24⁻40 yrs.) and older (75⁻90 yrs.) women and young (6⁻10 yrs.) and older (25⁻30 yrs.) rhesus macaques to define the role of circulating ceramides in the aging process. EVs were isolated using size-exclusion chromatography. Proteomic analysis was used to validate known exosome markers from Exocarta and nanoparticle tracking analysis used to characterize particle size and concentration. Specific ceram"],"journal":["Cells"],"pubmed_title":["Very Long-Chain C24:1 Ceramide Is Increased in Serum Extracellular Vesicles with Aging and Can Induce Senescence in Bone-Derived Mesenchymal Stem Cells."],"pmcid":["PMC6356348"],"funding_grant_id":["AG036675"],"pubmed_authors":["Khayrullin A","Liu Y","Fulzele S","Mattison JA","Hamrick MW","Mendhe B","Martinez-Nater L","Krishnan P"],"additional_accession":[]},"is_claimable":false,"name":"Very Long-Chain C24:1 Ceramide Is Increased in Serum Extracellular Vesicles with Aging and Can Induce Senescence in Bone-Derived Mesenchymal Stem Cells.","description":"Extracellular vesicles (EVs), including exosomes and microvesicles, function in cell-to-cell communication through delivery of proteins, lipids and microRNAs to target cells via endocytosis and membrane fusion. These vesicles are enriched in ceramide, a sphingolipid associated with the promotion of cell senescence and apoptosis. We investigated the ceramide profile of serum exosomes from young (24⁻40 yrs.) and older (75⁻90 yrs.) women and young (6⁻10 yrs.) and older (25⁻30 yrs.) rhesus macaques to define the role of circulating ceramides in the aging process. EVs were isolated using size-exclusion chromatography. Proteomic analysis was used to validate known exosome markers from Exocarta and nanoparticle tracking analysis used to characterize particle size and concentration. Specific ceram","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2026-07-09T12:10:12.076Z","creation":"2019-03-26T22:50:37Z"},"accession":"S-EPMC6356348","cross_references":{"pubmed":["30634626"],"doi":["10.3390/cells8010037"]}}