{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gomes Dos Reis L"],"funding":["Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","Australian Research Council"],"pagination":["E12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6359528"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["The use of cell-penetrating peptides (CPPs) in combination with nanoparticles (NPs) shows great potential for intracellular delivery of DNA. Currently, its application is limited due to the potential toxicity and unknown long-term side effects. In this study NPs prepared using a biodegradable polymer, poly(lactic⁻<i>co</i>⁻glycolic acid (PLGA) in association with a CPP, was assessed on two lung epithelial cell lines (adenocarcinomic human alveolar basal epithelial cells (A549) and normal bronchial epithelial cells (Beas-2B cells)). Addition of CPP was essential for intracellular internalization. No effects were observed on the mitochondrial activity and membrane integrity. Cells exposed to the NPs⁻DNA⁻CPP showed low inflammatory response, low levels of apoptosis and no activation of caspas"],"journal":["Pharmaceutics"],"pubmed_title":["Nanotoxicologic Effects of PLGA Nanoparticles Formulated with a Cell-Penetrating Peptide: Searching for a Safe pDNA Delivery System for the Lungs."],"pmcid":["PMC6359528"],"funding_grant_id":["LP140100181","99999.013454/2013-07"],"pubmed_authors":["Svolos M","Moir LM","Young PM","Gomes Dos Reis L","Windhab N","Lee WH","Jaber R","Traini D"],"additional_accession":[]},"is_claimable":false,"name":"Nanotoxicologic Effects of PLGA Nanoparticles Formulated with a Cell-Penetrating Peptide: Searching for a Safe pDNA Delivery System for the Lungs.","description":"The use of cell-penetrating peptides (CPPs) in combination with nanoparticles (NPs) shows great potential for intracellular delivery of DNA. Currently, its application is limited due to the potential toxicity and unknown long-term side effects. In this study NPs prepared using a biodegradable polymer, poly(lactic⁻<i>co</i>⁻glycolic acid (PLGA) in association with a CPP, was assessed on two lung epithelial cell lines (adenocarcinomic human alveolar basal epithelial cells (A549) and normal bronchial epithelial cells (Beas-2B cells)). Addition of CPP was essential for intracellular internalization. No effects were observed on the mitochondrial activity and membrane integrity. Cells exposed to the NPs⁻DNA⁻CPP showed low inflammatory response, low levels of apoptosis and no activation of caspas","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2025-04-19T07:52:09.444Z","creation":"2019-03-26T22:55:24Z"},"accession":"S-EPMC6359528","cross_references":{"pubmed":["30609825"],"doi":["10.3390/pharmaceutics11010012"]}}