biostudies-literatureUnknown2019Butler TAJAustralian Postgraduate Award Scholarship and Research Training SchemeDensitometry data generated for Western blots are commonly used to compare protein abundance between samples. In the last decade, it has become apparent that assumptions underpinning these comparisons are often violated in studies reporting Western blot data in the literature. These violations can lead to erroneous interpretations of data and may contribute to poor reproducibility of research. We assessed the reliability of Western blot data obtained to study human myometrial tissue proteins. We ran dilution series of protein lysates to explore the linearity of densitometry data. Proteins analysed included <i>α</i>SMA, HSP27, ERK1/2, and GAPDH. While ideal densitometry data are directly proportional to protein abundance, our data confirm that densitometry data often deviate from this idealBioMed research international5214821https://www.ebi.ac.uk/biostudies/studies/S-EPMC6360618biostudies-literatureMisleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures.PMC6360618Tolosa JMSmith RButler TAJChan ECPaul JWfalseMisleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures.Densitometry data generated for Western blots are commonly used to compare protein abundance between samples. In the last decade, it has become apparent that assumptions underpinning these comparisons are often violated in studies reporting Western blot data in the literature. These violations can lead to erroneous interpretations of data and may contribute to poor reproducibility of research. We assessed the reliability of Western blot data obtained to study human myometrial tissue proteins. We ran dilution series of protein lysates to explore the linearity of densitometry data. Proteins analysed included <i>α</i>SMA, HSP27, ERK1/2, and GAPDH. While ideal densitometry data are directly proportional to protein abundance, our data confirm that densitometry data often deviate from this ideal2019-01-01T00:00:00Z20192026-07-16T19:04:03.947Z2019-08-04T07:00:45ZS-EPMC63606183080067010.1155/2019/5214821